Overview

  • Product name
  • Description
    Rabbit polyclonal to CDK5RAP3
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to a region between residue 450 and the C-terminus (residue 506) of human CDK5RAP3 (NP_788276.1)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1.
    • Tissue specificity
      Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta.
    • Sequence similarities
      Belongs to the CDK5RAP3 family.
    • Post-translational
      modifications
      Phosphorylated in vitro by CDK5.
    • Information by UniProt
    • Database links
    • Alternative names
      • C53 antibody
      • CDK5 activator binding protein C53 antibody
      • CDK5 activator-binding protein C53 antibody
      • CDK5 regulatory subunit associated protein 3 antibody
      • CDK5 regulatory subunit-associated protein 3 antibody
      • Cdk5rap3 antibody
      • CK5P3_HUMAN antibody
      • HSF 27 antibody
      • HSF27 antibody
      • IC53 antibody
      • Ischemic heart CDK5 activator binding protein C53 antibody
      • LXXLL/leucine zipper containing ARFbinding protein antibody
      • LZAP antibody
      • MST016 antibody
      • OK/SW cl.114 antibody
      • PP1553 antibody
      • Protein HSF-27 antibody
      see all

    Images

    • All lanes : Anti-CDK5RAP3 antibody (ab70776) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 57 kDa
      Observed band size : 57 kDa
      Additional bands at : 220 kDa,28 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Detection of CDK5RAP3 by Western Blot of Immunprecipitate. ab70776 at 1µg/ml staining CDK5RAP3 in HeLa whole cell lysate immunoprecipitated using ab70776 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Chemiluminescence with exposure time of 30 seconds.

    References

    This product has been referenced in:
    • Horejší B  et al. Nuclear ?-tubulin associates with nucleoli and interacts with tumor suppressor protein C53. J Cell Physiol 227:367-82 (2012). Read more (PubMed: 21465471) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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