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Recombinant fragment: KDGLCRADQQ YECVAEIGEG AYGKVFKARD LKNGGRFVAL KRVRVQTGEE GMPLSTIREV AVLRHLETFE HPNVVRLFDV CTVSRTDRET KLTLVFE, corresponding to amino acids 3-100 of Human Cdk6
Our Abpromise guarantee covers the use of ab54576 in the following tested applications.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.|
Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 4 and 6: CDK6 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Green signal from target – ab54576 observed at 37 kDa
Lanes 3 and 4: Red signal from loading control – ab8227 observed at 42 kDa
Lanes 5 and 6: Merged (red and green) signal
ab54576 was shown to react with CDK6 when CDK6 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK6 knockout samples were subjected to SDS-PAGE. ab54576 and ab8227 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.