Overview

  • Product nameAnti-Cdk6 antibody
    See all Cdk6 primary antibodies
  • Description
    Mouse monoclonal to Cdk6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: KDGLCRADQQ YECVAEIGEG AYGKVFKARD LKNGGRFVAL KRVRVQTGEE GMPLSTIREV AVLRHLETFE HPNVVRLFDV CTVSRTDRET KLTLVFE, corresponding to amino acids 3-100 of Human Cdk6

Properties

Applications

Our Abpromise guarantee covers the use of ab54576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.

Target

  • FunctionProbably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDK 6 antibody
    • CDK6 antibody
    • CDK6_HUMAN antibody
    • Cell division protein kinase 6 antibody
    • Crk 2 antibody
    • Crk2 antibody
    • Cyclin dependent kinase 6 antibody
    • Cyclin-dependent kinase 6 antibody
    • MGC59692 antibody
    • p40 antibody
    • PLSTIRE antibody
    • Serine/threonine protein kinase PLSTIRE antibody
    • Serine/threonine-protein kinase PLSTIRE antibody
    • STQTL11 antibody
    see all

Anti-Cdk6 antibody images



  • Predicted band size : 37 kDa

    Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)

    Lanes 2, 4 and 6: CDK6 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Green signal from target – ab54576 observed at 37 kDa

    Lanes 3 and 4: Red signal from loading control – ab8227 observed at 42 kDa

    Lanes 5 and 6: Merged (red and green) signal

    ab54576 was shown to react with CDK6 when CDK6 knockout samples were used, along with additional cross-reactive bands. Wild-type and CDK6 knockout samples were subjected to SDS-PAGE. ab54576 and ab8227 (loading control to beta actin) were diluted 1 µg/mL and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.



  • Predicted band size : 37 kDa
    Cdk6 antibody (ab54576) at 1ug/lane + Jurkat cell lysate at 25ug/lane.

References for Anti-Cdk6 antibody (ab54576)

This product has been referenced in:
  • Hussain MS  et al. CDK6 associates with the centrosome during mitosis and is mutated in a large Pakistani family with primary microcephaly. Hum Mol Genet N/A:N/A (2013). Read more (PubMed: 23918663) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry.

Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. As such the concentration is set by these sources. All stock currently held by us does have a concentration of 0...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"