Anti-Cdk7 antibody (ab137716)
Key features and details
- Rabbit polyclonal to Cdk7
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cdk7 antibody
See all Cdk7 primary antibodies -
Description
Rabbit polyclonal to Cdk7 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Zebrafish -
Immunogen
Recombinant fragment corresponding to Human Cdk7 aa 1-346.
Database link: P50613 -
Positive control
- WB: Neuro2A, GL261, C8D30, NIH/3T3, BCL-1, Raw264.7, C2C12. HEK-293T, A431, A375. IP: HEK-293T. IHC-P: Human Breast cancer.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IP
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation of Ser-164 during mitosis inactivates the enzyme.
Phosphorylation of Thr-170 is required for activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1022 Human
- Entrez Gene: 12572 Mouse
- Omim: 601955 Human
- SwissProt: P50613 Human
- SwissProt: Q03147 Mouse
- Unigene: 184298 Human
- Unigene: 259718 Mouse
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Alternative names
- 39 kDa protein kinase antibody
- CAK antibody
- CAK1 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human Breast ca tissue labelling Cdk7 with ab137716 at 1/250.
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All lanes : Anti-Cdk7 antibody (ab137716) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa10% SDS-PAGE
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CDK7 was immunoprecipitated from 40μg of HEK-293T whole cell lysate with ab137716 at 1/500 dilution. EasyBlot anti-rabbit IgG was used as secondary antibody.
Lane 1: HEK-293T whole cell lysate 40μg (Input).
Lane 2: Control with 4 μg of preimmune Rabbit IgG.
Lane 3: Immunoprecipitation of CDK7 protein by 4 μg CDK7 antibody (ab137716). -
All lanes : Anti-Cdk7 antibody (ab137716) at 1/1000 dilution
Lane 1 : Neuro2A whole cell lysate
Lane 2 : GL261 whole cell lysate
Lane 3 : C8D30 whole cell lysate
Lane 4 : NIH/3T3 whole cell lysate
Lane 5 : BCL-1 whole cell lysate
Lane 6 : Raw264.7 whole cell lysate
Lane 7 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa10% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137716 has not yet been referenced specifically in any publications.