Overview

  • Product nameAnti-Cdk7 antibody
    See all Cdk7 primary antibodies
  • Description
    Rabbit polyclonal to Cdk7
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region between amino acids 330-346 of Human Cdk7.

  • Positive control
    • NIH3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 41.5 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionCyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
    Phosphorylation of Thr-170 is required for activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 39 kDa protein kinase antibody
    • CAK antibody
    • CAK1 antibody
    • Cdk activating kinase antibody
    • CDK-activating kinase 1 antibody
    • CDK-activating kinase antibody
    • cdk7 antibody
    • CDK7_HUMAN antibody
    • CDKN7 antibody
    • Cell division protein kinase 7 antibody
    • Cyclin dependent kinase 7 antibody
    • cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody
    • Cyclin-dependent kinase 7 antibody
    • HCAK antibody
    • Homolog of Xenopus MO15 Cdk activating kinase antibody
    • Kinase subunit of CAK antibody
    • MO15 antibody
    • MO15, Xenopus, homolog of antibody
    • P39 Mo15 antibody
    • p39MO15 antibody
    • Serine threonine kinase Stk1 antibody
    • Serine/threonine protein kinase 1 antibody
    • Serine/threonine protein kinase MO15 antibody
    • STK1 antibody
    • TFIIH basal transcription factor complex kinase subunit antibody
    see all

Anti-Cdk7 antibody images



  • Predicted band size : 41.5 kDa
    ab894 at 1/1000 dilution staining approximately 36kDa band of mouse cdk7 in NIH3T3 whole cell lysate by Western blot (ECL). ab894 at 1/1000 dilution staining approximately 36kDa band of mouse cdk7 in NIH3T3 whole cell lysate by Western blot (ECL).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling Cdk7 with ab894 at 4µg/ml. Staining in epithelial cells of intestinal villus.

  • Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml using anti-CDK7 antibody (ab894)

References for Anti-Cdk7 antibody (ab894)

ab894 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"