Overview

  • Product name
    Anti-Cdk7 (phospho T170) antibody
    See all Cdk7 primary antibodies
  • Description
    Rabbit polyclonal to Cdk7 (phospho T170)
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to amino acid residues surrounding Threonine 170 of human CDK7, conjugated to KLH

  • Positive control
    • Ramos tissue lysate; Human breast carcinoma tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/1000.

Target

  • Function
    Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
    Phosphorylation of Thr-170 is required for activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 39 kDa protein kinase antibody
    • CAK antibody
    • CAK1 antibody
    • Cdk activating kinase antibody
    • CDK-activating kinase 1 antibody
    • CDK-activating kinase antibody
    • cdk7 antibody
    • CDK7_HUMAN antibody
    • CDKN7 antibody
    • Cell division protein kinase 7 antibody
    • Cyclin dependent kinase 7 antibody
    • cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody
    • Cyclin-dependent kinase 7 antibody
    • HCAK antibody
    • Homolog of Xenopus MO15 Cdk activating kinase antibody
    • Kinase subunit of CAK antibody
    • MO15 antibody
    • MO15, Xenopus, homolog of antibody
    • P39 Mo15 antibody
    • p39MO15 antibody
    • Serine threonine kinase Stk1 antibody
    • Serine/threonine protein kinase 1 antibody
    • Serine/threonine protein kinase MO15 antibody
    • STK1 antibody
    • TFIIH basal transcription factor complex kinase subunit antibody
    see all

Images

  • Anti-Cdk7 (phospho T170) antibody (ab59987) at 1/100 dilution + Ramos tissue lysate

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue labelled with ab59987 at 1/50 dilution, followed by peroxidase conjugation to the secondary antibody and AEC staining.

References

This product has been referenced in:
  • Desai SR  et al. Regulation of Cdk7 activity through a phosphatidylinositol (3)-kinase/PKC-?-mediated signaling cascade in glioblastoma. Carcinogenesis 33:10-9 (2012). WB ; Human . Read more (PubMed: 22021906) »
  • Budhiraja S  et al. Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4T+ T cells and inhibits HIV-1 gene expression. Retrovirology 9:52 (2012). Read more (PubMed: 22727189) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7)
Loading amount
5 µg
Specification
MCF-7
Treatment
CDK7 Inhibitor
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Dean Heathcote

Verified customer

Submitted Oct 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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