Purification notesThis antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
1/50 - 1/100.
FunctionCyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylation of Ser-164 during mitosis inactivates the enzyme. Phosphorylation of Thr-170 is required for activity.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue labelled with ab59987 at 1/50 dilution, followed by peroxidase conjugation to the secondary antibody and AEC staining.
References for Anti-Cdk7 (phospho T170) antibody (ab59987)
This product has been referenced in:
Desai SR et al. Regulation of Cdk7 activity through a phosphatidylinositol (3)-kinase/PKC-?-mediated signaling cascade in glioblastoma. Carcinogenesis33:10-9 (2012).
Read more (PubMed: 22021906) »
Budhiraja S et al. Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4T+ T cells and inhibits HIV-1 gene expression. Retrovirology9:52 (2012).
Read more (PubMed: 22727189) »