Overview

  • Product nameAnti-Cdk7 (phospho T170) antibody
    See all Cdk7 primary antibodies
  • Description
    Rabbit polyclonal to Cdk7 (phospho T170)
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to amino acid residues surrounding Threonine 170 of human CDK7, conjugated to KLH

  • Positive control
    • Ramos tissue lysate; Human breast carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/1000.

Target

  • FunctionCyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
    Phosphorylation of Thr-170 is required for activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 39 kDa protein kinase antibody
    • CAK antibody
    • CAK1 antibody
    • Cdk activating kinase antibody
    • CDK-activating kinase 1 antibody
    • CDK-activating kinase antibody
    • cdk7 antibody
    • CDK7_HUMAN antibody
    • CDKN7 antibody
    • Cell division protein kinase 7 antibody
    • Cyclin dependent kinase 7 antibody
    • cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody
    • Cyclin-dependent kinase 7 antibody
    • HCAK antibody
    • Homolog of Xenopus MO15 Cdk activating kinase antibody
    • Kinase subunit of CAK antibody
    • MO15 antibody
    • MO15, Xenopus, homolog of antibody
    • P39 Mo15 antibody
    • p39MO15 antibody
    • Serine threonine kinase Stk1 antibody
    • Serine/threonine protein kinase 1 antibody
    • Serine/threonine protein kinase MO15 antibody
    • STK1 antibody
    • TFIIH basal transcription factor complex kinase subunit antibody
    see all

Anti-Cdk7 (phospho T170) antibody images

  • Anti-Cdk7 (phospho T170) antibody (ab59987) at 1/100 dilution + Ramos tissue lysate

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue labelled with ab59987 at 1/50 dilution, followed by peroxidase conjugation to the secondary antibody and AEC staining.

References for Anti-Cdk7 (phospho T170) antibody (ab59987)

This product has been referenced in:
  • Desai SR  et al. Regulation of Cdk7 activity through a phosphatidylinositol (3)-kinase/PKC-?-mediated signaling cascade in glioblastoma. Carcinogenesis 33:10-9 (2012). WB ; Human . Read more (PubMed: 22021906) »
  • Budhiraja S  et al. Phosphatase PPM1A negatively regulates P-TEFb function in resting CD4T+ T cells and inhibits HIV-1 gene expression. Retrovirology 9:52 (2012). Read more (PubMed: 22727189) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF-7)
Loading amount 5 µg
Specification MCF-7
Treatment CDK7 Inhibitor
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Dean Heathcote

Verified customer

Submitted Oct 16 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"