Overview

  • Product name
  • Description
    Rabbit polyclonal to Cdk8
  • Tested applications
    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 370-420 of Human Cdk8 (P49336).

  • Positive control
    • HeLa cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab115155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • cdk8 antibody
      • CDK8 protein kinase antibody
      • CDK8_HUMAN antibody
      • Cell Division Kinase 8 antibody
      • Cell division protein kinase 8 antibody
      • Cyclin Dependent kinase 8 antibody
      • Cyclin-dependent kinase 8 antibody
      • K35 antibody
      • Mediator complex subunit cdk8 antibody
      • Mediator of RNA polymerase II transcription subunit cdk8 antibody
      • Protein kinase K35 antibody
      see all

    Images

    • Immunoprecipitation of Cdk8 (3 µg of antibody per mg of HeLa cells lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdk8 antibody (ab115155); Lane 2-Immunoprecipitation with an anti-Cdk8 antibody that recognizes an upstream epitope; Lane 3- Immunoprecipitation with IgG control. An anti-Cdk8 antibody used in lane 2 was used at 0.4 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.

    References

    ab115155 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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