Overview

  • Product nameAnti-Cdk8 antibody
    See all Cdk8 primary antibodies
  • Description
    Rabbit polyclonal to Cdk8
  • Tested applicationsSuitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis , Medaka fish
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 370-420 of Human Cdk8 (P49336).

  • Positive control
    • HeLa cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab115155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P Use at an assay dependent concentration.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionComponent of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • cdk8 antibody
      • CDK8 protein kinase antibody
      • CDK8_HUMAN antibody
      • Cell Division Kinase 8 antibody
      • Cell division protein kinase 8 antibody
      • Cyclin Dependent kinase 8 antibody
      • Cyclin-dependent kinase 8 antibody
      • K35 antibody
      • Mediator complex subunit cdk8 antibody
      • Mediator of RNA polymerase II transcription subunit cdk8 antibody
      • Protein kinase K35 antibody
      see all

    Anti-Cdk8 antibody images

    • Immunoprecipitation of Cdk8 (3 µg of antibody per mg of HeLa cells lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdk8 antibody (ab115155); Lane 2-Immunoprecipitation with an anti-Cdk8 antibody that recognizes an upstream epitope; Lane 3- Immunoprecipitation with IgG control. An anti-Cdk8 antibody used in lane 2 was used at 0.4 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.

    References for Anti-Cdk8 antibody (ab115155)

    ab115155 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab115155.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"