Overview

  • Product nameAnti-Cdk8 antibody
    See all Cdk8 primary antibodies
  • Description
    Rabbit polyclonal to Cdk8
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: MouseDoes not react with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 244-272 of Mouse Cdk8 (NP_705827.2).

  • Positive control
    • K562 cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab123940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 53 kDa.

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • cdk8 antibody
    • CDK8 protein kinase antibody
    • CDK8_HUMAN antibody
    • Cell Division Kinase 8 antibody
    • Cell division protein kinase 8 antibody
    • Cyclin Dependent kinase 8 antibody
    • Cyclin-dependent kinase 8 antibody
    • K35 antibody
    • Mediator complex subunit cdk8 antibody
    • Mediator of RNA polymerase II transcription subunit cdk8 antibody
    • Protein kinase K35 antibody
    see all

Anti-Cdk8 antibody images

  • Anti-Cdk8 antibody (ab123940) at 1/100 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 53 kDa

References for Anti-Cdk8 antibody (ab123940)

ab123940 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryos)
Specification 0-4hr embryos
Treatment control shRNA and Cdk8 shRNA embryos
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Richelle Sopko

Verified customer

Submitted Mar 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"