Overview

  • Product nameAnti-Cdk8 antibody
    See all Cdk8 primary antibodies
  • Description
    Rabbit polyclonal to Cdk8
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Human) derived from the N-terminal of Cdk8.

  • Positive control
    • Extracts from 293 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • cdk8 antibody
    • CDK8 protein kinase antibody
    • CDK8_HUMAN antibody
    • Cell Division Kinase 8 antibody
    • Cell division protein kinase 8 antibody
    • Cyclin Dependent kinase 8 antibody
    • Cyclin-dependent kinase 8 antibody
    • K35 antibody
    • Mediator complex subunit cdk8 antibody
    • Mediator of RNA polymerase II transcription subunit cdk8 antibody
    • Protein kinase K35 antibody
    see all

Anti-Cdk8 antibody images

  • All lanes : Anti-Cdk8 antibody (ab64940) at 1/500 dilution

    Lane 1 : extracts from 293 cells (5-30µg total protein)
    Lane 2 : extracts from 293 cells (5-30µg total protein) and 5-10µg of the immunising peptide.


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Cdk8 antibody (ab64940)

ab64940 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"