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Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE)
Our Abpromise guarantee covers the use of ab6544 in the following tested applications.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21378166|
|ELISA||1/500 - 1/2000.|
|IHC-P||1/200 - 1/1000.|
|IP||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Predicted molecular weight: 43 kDa.|
Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.
Immunofluorescence analysis of HeLa cells, staining Cdk9 (red) with ab6544.
The cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100 for 10 min at 4°C. The cells were incubated with 0.05% PBS-T before incubating with primary antibody at 1/2000 dilution.