Overview

  • Product name
    Anti-Cdk9 antibody [EP3118]
    See all Cdk9 primary antibodies
  • Description
    Rabbit monoclonal [EP3118] to Cdk9
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in human Cdk9

  • Positive control
    • HT-29, HeLa and 293T cell lysates. Human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/2000 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

    Is unsuitable for Flow Cyt, ICC or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-2K antibody
      • CDC2 related kinase antibody
      • CDC2L4 antibody
      • Cdk 9 antibody
      • Cdk9 antibody
      • CDK9_HUMAN antibody
      • Cell division cycle 2-like protein kinase 4 antibody
      • Cell division protein kinase 9 antibody
      • CTK1 antibody
      • Cyclin dependent kinase 9 antibody
      • Cyclin-dependent kinase 9 antibody
      • PITALRE antibody
      • Serine/threonine-protein kinase PITALRE antibody
      • TAK antibody
      • Tat associated kinase complex catalytic subunit antibody
      see all

    Images

    • ab75848 at 1/100 dilution staining Cdk9 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.
    • All lanes : Anti-Cdk9 antibody [EP3118] (ab75848) at 1/2000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 43 kDa
      Observed band size : 43 kDa

    References

    This product has been referenced in:
    • Izumi K  et al. Germline gain-of-function mutations in AFF4 cause a developmental syndrome functionally linking the super elongation complex and cohesin. Nat Genet 47:338-44 (2015). Read more (PubMed: 25730767) »
    • Chen XX  et al. Cyclin-dependent kinase inhibitor dinaciclib potently synergizes with cisplatin in preclinical models of ovarian cancer. Oncotarget 6:14926-39 (2015). Read more (PubMed: 25962959) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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