• Product nameAnti-Cdk9 antibody [EP3118]
    See all Cdk9 primary antibodies
  • Description
    Rabbit monoclonal [EP3118] to Cdk9
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in human Cdk9

  • Positive control
    • HT-29, HeLa and 293T cell lysates. Human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab75848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
    WB: 1/2000 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

    Is unsuitable for Flow Cyt, ICC or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-2K antibody
      • CDC2 related kinase antibody
      • CDC2L4 antibody
      • Cdk 9 antibody
      • Cdk9 antibody
      • CDK9_HUMAN antibody
      • Cell division cycle 2-like protein kinase 4 antibody
      • Cell division protein kinase 9 antibody
      • CTK1 antibody
      • Cyclin dependent kinase 9 antibody
      • Cyclin-dependent kinase 9 antibody
      • PITALRE antibody
      • Serine/threonine-protein kinase PITALRE antibody
      • TAK antibody
      • Tat associated kinase complex catalytic subunit antibody
      see all

    Anti-Cdk9 antibody [EP3118] images

    • ab75848 at 1/100 dilution staining Cdk9 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.
    • All lanes : Anti-Cdk9 antibody [EP3118] (ab75848) at 1/2000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 43 kDa
      Observed band size : 43 kDa

    References for Anti-Cdk9 antibody [EP3118] (ab75848)

    ab75848 has not yet been referenced specifically in any publications.

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