Recombinant
RabMAb

Anti-Cdk9 antibody [EPR3119Y] (ab76320)

Overview

  • Product name
    Anti-Cdk9 antibody [EPR3119Y]
    See all Cdk9 primary antibodies
  • Description
    Rabbit monoclonal [EPR3119Y] to Cdk9
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminus of Human Cdk9

  • Positive control
    • WB: HT-29, HL-60, HeLa or 293 cell lysates. IHC-P: Human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 43 kDa.
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-2K antibody
      • CDC2 related kinase antibody
      • CDC2L4 antibody
      • Cdk 9 antibody
      • Cdk9 antibody
      • CDK9_HUMAN antibody
      • Cell division cycle 2-like protein kinase 4 antibody
      • Cell division protein kinase 9 antibody
      • CTK1 antibody
      • Cyclin dependent kinase 9 antibody
      • Cyclin-dependent kinase 9 antibody
      • PITALRE antibody
      • Serine/threonine-protein kinase PITALRE antibody
      • TAK antibody
      • Tat associated kinase complex catalytic subunit antibody
      see all

    Images

    • All lanes : Anti-Cdk9 antibody [EPR3119Y] (ab76320) at 1/2000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HL-60 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 43 kDa
    • ab76320 (1/200) staining Cdk9 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab76320 at 1/100 dilution.
    • ab76320 showing positive staining in Cervical carcinoma tissue.

    • ab76320 showing positive staining in Colonic adenocarcinoma tissue.

    • ab76320 showing positive staining in Lung adenocarcinoma tissue.

    • ab76320 showing positive staining in Normal tonsil tissue.

    References

    This product has been referenced in:
    • Polioudakis D  et al. MiR-191 Regulates Primary Human Fibroblast Proliferation and Directly Targets Multiple Oncogenes. PLoS One 10:e0126535 (2015). WB . Read more (PubMed: 25992613) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (HAP1 cells)
    Negative control
    1kb up and downstream of the TSS of the hnRNP H1 gene, an untranscribed region on chr.17 (q21.31) and 2,7 kb downstream of the TES of the Gadd45b gene.
    Specification
    HAP1 cells
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: formaldehyde
    Positive control
    hnRNP H1, b-actin, GAPDH and myc gene promoters
    Username

    Abcam user community

    Verified customer

    Submitted Sep 12 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Oct 19 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - whole cell (Xenopus laevis gastrula embryo extract (Stage12))
    Loading amount
    0.5 µg
    Specification
    Xenopus laevis gastrula embryo extract (Stage12)
    Gel Running Conditions
    Reduced Denaturing (12% SDS-PAGE)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
    Username

    Dr. Kei Miyamoto

    Verified customer

    Submitted Oct 10 2012

    Thank you very much for your interest in our products.

    We have three antibodies which may interest you as they are all tested for both IP and WB.In addition,the homology between the two species is 100% for this protein, therefore,it seems ver...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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