Overview

  • Product nameAnti-CDKAL1 antibody
    See all CDKAL1 primary antibodies
  • Description
    Rabbit polyclonal to CDKAL1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human CDKAL1.

    (Peptide available as ab74018.)

  • Positive control
    • This antibody gave a positive signal in the following human whole cell lysates: HepG2 and U2OS

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

Target

  • FunctionCatalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.
  • Tissue specificityExpressed in pancreatic islets.
  • Involvement in diseaseGenetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similaritiesBelongs to the methylthiotransferase family. CDKAL1 subfamily.
    Contains 1 MTTase N-terminal domain.
    Contains 1 TRAM domain.
  • Cellular localizationEndoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDK5 regulatory subunit associated protein 1 like 1 antibody
    • CDK5 regulatory subunit-associated protein 1-like 1 antibody
    • CDKAL_HUMAN antibody
    • CDKAL1 antibody
    • Threonylcarbamoyladenosine tRNA methylthiotransferase antibody
    • tRNA t 6 A37 methylthiotransferase antibody
    • tRNA-t(6)A37 methylthiotransferase antibody
    see all

Anti-CDKAL1 antibody images

  • All lanes : Anti-CDKAL1 antibody (ab68045) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,26 kDa,280 kDa,38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab68045 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • All lanes : Anti-CDKAL1 antibody (ab68045) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • All lanes : Anti-CDKAL1 antibody (ab68045) at 1 µg/ml

    Lane 1 : Human Jurkat whole cell lysate at 20 µg
    Lane 2 : Mouse MEF whole cell lysate at 20 µg
    Lane 3 : Mouse 3T3 whole cell lysate at 20 µg
    Lane 4 : Mouse GC-1 whole cell lysate at 20 µg

    Secondary
    HRP-conjugated swine anti-rabbit polyclonal at 1/5000 dilution

    Predicted band size : 65 kDa

    This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.

    See Abreview

References for Anti-CDKAL1 antibody (ab68045)

This product has been referenced in:
  • Okamura T  et al. Deletion of CDKAL1 affects high-fat diet-induced fat accumulation and glucose-stimulated insulin secretion in mice, indicating relevance to diabetes. PLoS One 7:e49055 (2012). WB ; Mouse . Read more (PubMed: 23173044) »
  • Ohara-Imaizumi M  et al. Deletion of CDKAL1 affects mitochondrial ATP generation and first-phase insulin exocytosis. PLoS One 5:e15553 (2010). WB ; Mouse . Read more (PubMed: 21151568) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Jurkat, MEF, 3T3, GC-1)
Loading amount 20 µg
Specification Jurkat, MEF, 3T3, GC-1
Gel Running Conditions Reduced Denaturing (Pierce Precise Protein Gel 4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

Submitted Jan 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"