Overview

  • Product nameAnti-CDKL5 antibody
    See all CDKL5 primary antibodies
  • Description
    Rabbit polyclonal to CDKL5
  • SpecificitySwissprot predicts mouse CDKL5 protein to be ~105 kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118 kDa on WB. On this basis we think that the ~120 kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120 kDa band is blocked by the immunising peptide. The larger ~200 kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human CDKL5.

    (Peptide available as ab25756.)

  • Positive control
    • adult mouse brain

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab22453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 120,160 kDa (predicted molecular weight: 105 kDa).
IHC-P 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr 1/300.

Target

  • FunctionMediates phosphorylation of MECP2.
  • Tissue specificityExpressed in brain, lung, kidney, prostate, ovary, placenta, pancreas and testis.
  • Involvement in diseaseNote=Chromosomal aberrations involving CDKL5 are found in patients manifesting early-onset seizures and spams and psychomotor impairment. Translocation t(X;6)(p22.3;q14); translocation t(X;7)(p22.3;p15).
    Defects in CDKL5 are a cause of epileptic encephalopathy early infantile type 2 (EIEE2) [MIM:300672]; also known as atypical CDKL5-related Rett syndrome. EIEE2 is a severe form of epilepsy characterized by seizures or spasms beginning in infancy. Patients manifest features resembling Rett syndrome such as microcephaly, lack of speech development, stereotypic hand movements.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cdkl5 antibody
    • CDKL5_HUMAN antibody
    • Cyclin dependent kinase 5 transcript antibody
    • Cyclin-dependent kinase-like 5 antibody
    • EIEE2 antibody
    • ISSX antibody
    • Serine/threonine kinase 9 antibody
    • Serine/threonine-protein kinase 9 antibody
    • Stk9 antibody
    see all

Anti-CDKL5 antibody images

  • All lanes : Anti-CDKL5 antibody (ab22453) at 3 µg/ml

    Lane 1 : mouse cortex
    Lane 2 : mouse cortex with Human CDKL5 peptide (ab25756) at 2 µg/ml

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 105 kDa
    Observed band size : 120,200 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes

    This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

    Swissprot predicts mouse CDKL5 protein to be ~105kDa (Q3UTQ8), however a recent paper by Lin et al, 2006 (PMID: 16330482) indicated CDKL5 to run at ~118kDa on WB. On this basis we think that the ~120kDa band we observe using Rabbit polyclonal to CDKL5 (ab22453) on mouse tissue is CDKL5 specific. Futhermore the ~120kDa band is blocked by the immunising peptide. The larger >150kDa is potentially also CDKL5 as a similar band is also observed in the Lin et al (2006) publication. There is evidence for larger forms of CDKL5 for the human version of the protein, so it may not be surprising to find a similar scenario for the mouse protein.
  • Image courtesy of Human Protein Atlas

    ab22453 staining nuclear CDKL5 in Human appendix. The paraffin embedded human skin tissue was incubated with ab22453 (1/800 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab22453 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

    Further results for this antibody can be found at www.proteinatlas.org

  • IHC-FoFr image of CDKL5 staining using ab22453(1:300) on Mouse hippocampus sections. The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

References for Anti-CDKL5 antibody (ab22453)

This product has been referenced in:
  • Wang IT  et al. Loss of CDKL5 disrupts kinome profile and event-related potentials leading to autistic-like phenotypes in mice. Proc Natl Acad Sci U S A : (2012). WB . Read more (PubMed: 23236174) »
  • Nemos C  et al. Mutational spectrum of CDKL5 in early-onset encephalopathies: a study of a large collection of French patients and review of the literature. Clin Genet 76:357-71 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19793311) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain - hippocampus)
Specification Brain - hippocampus
Username

Dr. Sophie Pezet

Verified customer

Submitted May 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"