Anti-CDKN2A/p16INK4a antibody [DCS50.1] (ab16123)

Overview

• Product nameAnti-CDKN2A/p16INK4a antibody [DCS50.1]See all CDKN2A/p16INK4a primary antibodies ...
• Description
  Mouse monoclonal [DCS50.1] to CDKN2A/p16INK4a
• SpecificityThis antibody shws no cross reactivity with the closely related inhibitors p15INK4b and p18INK4c.
• Tested applicationsICC/IF, Sandwich ELISA, WB, IP, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein (Human).

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.08% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• Purification notesThis antibody is provided as a 0.2µm sterile filtered solution.
• Clonality Monoclonal
• Clone numberDCS50.1
• IsotypeIgG1
• Research Areas
  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Ink4

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • INK4a/Arf

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Ink4

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Ink4

Applications

Target

• RelevanceThe CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16(INK4A) and p14(ARF). This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
• Cellular localizationCytoplasmic and Nuclear
• Database links
  • Entrez Gene: 1029 Human
  • Omim: 600160 Human
  • SwissProt: P42771 Human
  • Unigene: 512599 Human

• Alternative names
  ARF antibodyCCM2 antibodyCDK4 Inhibitor antibody

  CDK4 inhibitor p16 INK4 antibodyCDK4I antibodyCDKN2 antibodyCDKN2A antibodyCell cycle negative regulator beta antibodyCMM2 antibodyCyclin dependent kinase 4 inhibitor A antibodyCyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibodyCyclin Dependent Kinase Inhibitor 2A antibodyCyclin dependent kinase inhibitor 2A isoform 4 antibodyCyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibodyCyclin dependent kinase inhibitor p16 antibodyINK4 antibodyINK4 antibodyINK4A antibodyMLM antibodyMTS1 antibodyMultiple tumor suppressor 1 antibodyMultiple Tumor Supressor 1 antibodyp14 antibodyp14(ARF) antibodyp14ARF antibodyp16 antibodyp16 INK4 antibodyp16INK4 antibodyP16INK4 antibodyp16INK4a antibodyp19 antibodyp19(ARF) antibodyp19Arf antibodyTP16 antibody

  see all

Anti-CDKN2A/p16INK4a antibody [DCS50.1] images

• 

  Sandwich ELISA - CDKN2A/p16INK4a antibody [DCS50.1] (ab16123)
  Standard Curve for CDKN2A/p16INK4a (Analyte: CDKN2A/p16INK4a protein (Human) (ab84075)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [DCS50.1] to CDKN2A/p16INK4a (ab16123) at 1µg/ml and Detector Antibody Rabbit polyclonal to CDKN2A/p16INK4a (ab7962) at 0.5µg/ml.
• 

  Immunocytochemistry/ Immunofluorescence-CDKN2A/p16INK4a antibody [DCS50.1](ab16123)
  ICC/IF image of ab16123 stained Hepp cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Western blot - CDKN2A/p16INK4a antibody [DCS50.1] (ab16123)
  All lanes : Anti-CDKN2A/p16INK4a antibody [DCS50.1] (ab16123) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 16 kDa
  Observed band size : 16 kDa
  Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 12 minutes
• 

  Flow Cytometry - CDKN2A/p16INK4a antibody [DCS50.1] (ab16123)
  Overlay histogram showing HEK293 cells stained with ab16123 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16123, 1µg/1x10⁶ cells ) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol used under the same conditions.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  

Protocols

Protocol Booklet