Overview

  • Product nameAnti-CDKN2AIP antibody
    See all CDKN2AIP primary antibodies
  • Description
    Mouse monoclonal to CDKN2AIP
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human CDKN2AIP protein (AAH22270) with a 26 kDa proprietary tag.

  • Positive control
    • ICC/IF: HeLa cells WB: HeLa nuclear extract

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 61 kDa.
ICC/IF Use a concentration of 25 µg/ml.
ELISA Use at an assay dependent dilution. Detection limit for ab88322 is approximately 1 ng/ml when used as a capture antibody.

Target

  • FunctionActivates p53/TP53 by CDKN2A-dependent and CDKN2A-independent pathways.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the CARF family.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
  • Post-translational
    modifications
    May be ubiquitinated.
  • Cellular localizationNucleus > nucleoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calcium response factor antibody
    • CARF antibody
    • CARF_HUMAN antibody
    • CDKN2A interacting protein antibody
    • CDKN2A-interacting protein antibody
    • Cdkn2aip antibody
    • collaborates/cooperates with ARF (alternate reading frame) protein antibody
    • Collaborator of ARF antibody
    • FLJ20036 antibody
    see all

Anti-CDKN2AIP antibody images

  • Anti-CDKN2AIP antibody (ab88322) at 5 µg/ml + immunogen (recombinant human CDKN2AIP fragment with a 26 kDa proprietary tag) at 0.2 µg/ml

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 61 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • Anti-CDKN2AIP antibody (ab88322) at 5 µg/ml + HeLa nuclear extract at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 61 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)
  • ab88322, at 25 µg/ml, staining CDKN2AIP in HeLa cell by Immunofluorescence.

References for Anti-CDKN2AIP antibody (ab88322)

This product has been referenced in:
  • Polioudakis D  et al. A Myc-microRNA network promotes exit from quiescence by suppressing the interferon response and cell-cycle arrest genes. Nucleic Acids Res 41:2239-54 (2013). Read more (PubMed: 23303785) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"