Overview

  • Product nameAnti-CDKN2A/p14ARF antibody
    See all CDKN2A/p14ARF primary antibodies
  • Description
    Rabbit polyclonal to CDKN2A/p14ARF
  • SpecificitySpecific for p14ARF.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Positive control
    • BT549 cells, HeLa whole cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 14 kDa).
ICC/IF 1/100.

Target

  • RelevanceThe gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • ARF antibody
    • CDK4 inhibitor p16 INK4 antibody
    • CDK4I antibody
    • CDKN2 antibody
    • CDKN2A antibody
    • Cell cycle negative regulator beta antibody
    • CMM2 antibody
    • Cyclin dependent kinase 4 inhibitor A antibody
    • Cyclin dependent kinase inhibitor 2A antibody
    • INK4 antibody
    • INK4a antibody
    • Melanoma p16 inhibits CDK4 antibody
    • MLM antibody
    • MTS 1 antibody
    • MTS1 antibody
    • Multiple tumor suppressor 1 antibody
    • p14 antibody
    • p16 antibody
    • p16 INK4a antibody
    • p16INK4a antibody
    • p19 antibody
    • P19ARF antibody
    • TP16 antibody
    see all

Anti-CDKN2A/p14ARF antibody images

  • ab470 diluted 1:267 (7.5 µg/ml) and used on NARF6 cells induced with 1 mM IPTG for 24 hrs to induce p14 ARF.



  • Predicted band size : 14 kDa

    ab470 used at 1 µg/ml with ECL on human cells transfected with p14ARF construct.

    ab470 used at 1 µg/ml with ECL on human cells transfected with p14ARF construct.
  • All lanes : Anti-CDKN2A/p14ARF antibody (ab470) at 1/400 dilution

    Lane 1 : Lysate from ARF negative U2OS cells
    Lane 2 : Lysate from ARF positive H1299 cells


    Predicted band size : 14 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of Delin Chen

    The picture shows Western blot using cell lysate from U2OS cells (ARF negative cells, lane 1)  and H1299 cells (ARF positive cells, lane 2) stained with ab470 at a dilution of 1/400.

    This image was kindly supplied as part of the review submitted by Delin Chen.

References for Anti-CDKN2A/p14ARF antibody (ab470)

This product has been referenced in:
  • Rao RA  et al. Ezh2 mediated H3K27me3 activity facilitates somatic transition during human pluripotent reprogramming. Sci Rep 5:8229 (2015). Human . Read more (PubMed: 25648270) »
  • Wei J  et al. Bacterial CagA protein induces degradation of p53 protein in a p14ARF-dependent manner. Gut N/A:N/A (2014). Human . Read more (PubMed: 25080447) »

See all 8 Publications for this product

Product Wall

Thank you for your enquiry. Our source for this antibody has told me that ab11048 does detect endogenous p14ARF; please see the following publication for additional information: Llanos S et al. Stabilization of p53 by p14ARF without relocation of MD...

Read More

Thank you for your patience. Regarding ab5953, unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

Ab470 was tested by Western analysis on HeLa (weak signal at 1:500) and BT549 cells (strong signal at 1:1,000+). IF was done on BT549 cells.

This antibody has not been tested in IP, therefore we cannot guarantee results.

It is not known. Since it has been used for IF at 1:100 it may but at a low dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"