Anti-CDKN2A/p16INK4a antibody [1E12E10] (ab117443)

Overview

  • Product nameAnti-CDKN2A/p16INK4a antibody [1E12E10]
    See all CDKN2A/p16INK4a primary antibodies
  • Description
    Mouse monoclonal [1E12E10] to CDKN2A/p16INK4a
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human CDKN2A/p16INK4a aa 1-200. Ni-NTA purified. Expressed in E. Coli strain BL21 (DE3).
    Database link: NP_000068.1

  • Positive control
    • Human colon (crypt) tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab117443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/2000. Predicted molecular weight: 17 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • FormThere are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
  • Alternative names
    • CCM2 antibody
    • CDK4 inhibitor p16 INK4 antibody
    • CDK4I antibody
    • CDKN2 antibody
    • CDKN2A antibody
    • Cell cycle negative regulator beta antibody
    • CMM2 antibody
    • Cyclin dependent kinase 4 inhibitor A antibody
    • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
    • Cyclin Dependent Kinase Inhibitor 2A antibody
    • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
    • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
    • Cyclin dependent kinase inhibitor p16 antibody
    • INK4 antibody
    • INK4A antibody
    • MLM antibody
    • MTS1 antibody
    • Multiple tumor suppressor 1 antibody
    • p14 antibody
    • p16 antibody
    • P16INK4 antibody
    • p16INK4a antibody
    • p19 antibody
    • p19Arf antibody
    • TP16 antibody
    see all

Anti-CDKN2A/p16INK4a antibody [1E12E10] images

  • ab117443, at 5 µg/ml, staining CDKN2A/p14ARF in Formalin-fixed, Paraffin-embedded Human Colon (crypt) tissue by Immunohistochemistry.

References for Anti-CDKN2A/p16INK4a antibody [1E12E10] (ab117443)

ab117443 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse Colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Specification Mouse Colon
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde
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Submitted Sep 14 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Specification skin
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 14 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"