Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Ink4
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ab54210 has been referenced in 3 publications.
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All lanes : Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210) at 1/500 dilution
Lane 1 : Molecular weight marker
Lane 2 : Truncated p16 recombinant protein
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Ab54210 (1:500) staining p16 INK4a in rat liver tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.
Ab54210 (1:500) staining p16 INK4a in human brain tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.
Ab54210 (1:500) staining p16 INK4a in human brain tumor tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.
Overlay histogram showing HeLa cells stained with ab54210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54210, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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