Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)

Overview

  • Product nameAnti-CDKN2A/p16INK4a antibody [2D9A12]
    See all CDKN2A/p16INK4a primary antibodies
  • Description
    Mouse monoclonal [2D9A12] to CDKN2A/p16INK4a
  • Tested applicationsSuitable for: Flow Cyt, ELISA, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human CDKN2A/p16INK4a.
    Database link: P42771

  • Positive control
    • Rat liver tissue; Human brain tissue; Human brain tumor tissue; PFA/Tween HeLa cells.
  • General notes

    We have received positive and negative feedback from customers for ICC/IF. ICC/IF is not a batch-tested application for this antibody and we are not able to cover it under our Abpromise guarantee.

Properties

Applications

Our Abpromise guarantee covers the use of ab54210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA 1/10000.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Recommended antigen retrieval: Place slides in a special glass slide holder and fill in the rack with slides to ensure even heating. Rinse rack in 1L of Citrate buffer in ordinary pressure cooker,(0.01M,pH6.0) to immerse all slides. Cover the cap and heat for 15-20 minutes on electric cooker until water boils. Begin timing, only 2 minutes in high-pressure Remove the pressure cooker, Allow it to cool(Cool the slides at room temperature) Rinse the slides in PBS for 5 minutes
  • Application notesIs unsuitable for WB.
  • Target

    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • FormThere are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
    • Alternative names
      • CCM2 antibody
      • CDK4 inhibitor p16 INK4 antibody
      • CDK4I antibody
      • CDKN2 antibody
      • CDKN2A antibody
      • Cell cycle negative regulator beta antibody
      • CMM2 antibody
      • Cyclin dependent kinase 4 inhibitor A antibody
      • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
      • Cyclin Dependent Kinase Inhibitor 2A antibody
      • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
      • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
      • Cyclin dependent kinase inhibitor p16 antibody
      • INK4 antibody
      • INK4A antibody
      • MLM antibody
      • MTS1 antibody
      • Multiple tumor suppressor 1 antibody
      • p14 antibody
      • p16 antibody
      • P16INK4 antibody
      • p16INK4a antibody
      • p19 antibody
      • p19Arf antibody
      • TP16 antibody
      see all

    Anti-CDKN2A/p16INK4a antibody [2D9A12] images

    •  ab54210 (1:500) staining p16 INK4a in human brain tumor tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.

    • ab54210 staining CDKN2A/p16INK4a in Human cervix and ovarian carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/4000 in Dako diluent) for 20 minutes at 25°C. An undiluted HRP-conjugated Mouse polymer was used as the secondary antibody.

      See Abreview

    • ab54210 staining CDKN2A/p16INK4a in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 1 hour at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 1% NGS/TBS) for 20 hours at 4°C. Ab6785 (1/800) was used as the secondary antibody.

      See Abreview

    • ab54210 (1:500) staining p16 INK4a in rat liver tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.

    • Overlay histogram showing HeLa cells stained with ab54210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54210, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    •  ab54210 (1:500) staining p16 INK4a in human brain tissue by immunohistochemistry using paraffin embedded tissue. Nuclear staining (by DAB staining) is shown.

    References for Anti-CDKN2A/p16INK4a antibody [2D9A12] (ab54210)

    This product has been referenced in:
    • Hsu CK  et al. Bowen's disease with features resembling myrmecia wart. J Dermatol 42:90-3 (2015). Read more (PubMed: 25387725) »
    • Maeda M  et al. Intermittent high glucose implements stress-induced senescence in human vascular endothelial cells: role of superoxide production by NADPH oxidase. PLoS One 10:e0123169 (2015). Human . Read more (PubMed: 25879533) »

    See all 14 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (Inguinal Adipose Tissue)
    Gel Running Conditions Reduced Denaturing (2-20% Precast Gel, 100V for 1 hr 20 min to run)
    Loading amount 40 µg
    Specification Inguinal Adipose Tissue
    Blocking step SuperBlock T20 (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 13 2016

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Brain (medulloblastoma preneoplastic lesions))
    Permeabilization Yes - 0.3% Triton
    Specification Brain (medulloblastoma preneoplastic lesions)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative Paraformaldehyde
    Username

    Lukas Tamayo

    Verified customer

    Submitted Jun 20 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (brain section)
    Permeabilization Yes - 0.8% Triton-100 in PBS
    Specification brain section
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Fixative Paraformaldehyde
    Username

    Peisu Zhang

    Verified customer

    Submitted Mar 15 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (vascular smooth muscle cell)
    Permeabilization Yes - NP40
    Specification vascular smooth muscle cell
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jan 06 2016

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (15%gel)
    Sample Mouse Tissue lysate - whole (hippocampus)
    Specification hippocampus
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 10 2015



    CDKN2A/p16INK4a est une protéine qui est exprimé dans 63% de tumeurs et non pas que dans les tumeurs cérébrales. Vous pouvez donc également utiliser d’autres tissus cancéreux comme contrôl...

    Read More

    Thank you for contacting us.

    Human brain slides, such as ab4304, or humor brain tumor slides such as ab4679 should be good positive controls.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need ...

    Read More

    Thank you for contacting us.

    Here are the links to the Human Protein Atlas, including ones to the sites for normal and cancer tissue as well as cell lines. Several brain cell lines are listed as expressing CDKN2A in moderate levels.

    h...

    Read More

    Thank you for contacting Abcam and it was good to meet you at the Distributor Day events.

    I have looked through our catalogue to try and find some antibodies that would match your customers needs and I have placed links to the best matches bel...

    Read More

    Thank you for your enquiry and your interest in our products.
    We have several antibodies against CDKN2A but none of them have been tested for cross-reactivity with Syrian hamster (Mesocricetus auratus).
    Regarding ab54210 - the exact immunogen...

    Read More

    1-10 of 26 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"