Anti-CDKN2A/p16INK4a antibody (ab6546)

Overview

  • Product nameAnti-CDKN2A/p16INK4a antibody
    See all CDKN2A/p16INK4a primary antibodies
  • Description
    Rabbit polyclonal to CDKN2A/p16INK4a
  • SpecificityCross reactivity may occur with p15 when immunoprecipitating in vitro translated product but not in immunoblots. Potentially cross reacts with p15.5.
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    human p16 protein (missing the first 7 amino acids)

  • Positive control
    • Saos-2 lysate
  • General notesp16 (also known as p16 -INK4a, p16-MTS1, inhibitor of CDK4), is the product of the CDKN2 gene. It inhibits the progression of cell cycle through the G1 phase, by competing with D type cyclins to bind to CDK4 and CDK6. p16INK4/CDKN2is a candidate tumor suppressor, whose gene is frequently deleted or mutated in diverse types of cancer.

Properties

Applications

Our Abpromise guarantee covers the use of ab6546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: 1/1000. Predicted molecular weight: 17 kDa.
    This antisera is also suitable for immmunoprecipitation of in vitro translated protein and cell lysates (Saos-2, 3T3-L1 weakly) and will co-precipitate associated proteins including p15 and potentially p15.5.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • FormThere are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
    • Alternative names
      • CCM2 antibody
      • CDK4 inhibitor p16 INK4 antibody
      • CDK4I antibody
      • CDKN2 antibody
      • CDKN2A antibody
      • Cell cycle negative regulator beta antibody
      • CMM2 antibody
      • Cyclin dependent kinase 4 inhibitor A antibody
      • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
      • Cyclin Dependent Kinase Inhibitor 2A antibody
      • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
      • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
      • Cyclin dependent kinase inhibitor p16 antibody
      • INK4 antibody
      • INK4A antibody
      • MLM antibody
      • MTS1 antibody
      • Multiple tumor suppressor 1 antibody
      • p14 antibody
      • p16 antibody
      • P16INK4 antibody
      • p16INK4a antibody
      • p19 antibody
      • p19Arf antibody
      • TP16 antibody
      see all

    References for Anti-CDKN2A/p16INK4a antibody (ab6546)

    ab6546 has not yet been referenced specifically in any publications.

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    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below;

    http://www.abcam.com/ps/pdf/protocols/Sandwich%20ELISA.pdf

    http://www.abca...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"