Overlay histogram showing HEK293 cells stained with ab81278 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81278, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-CDKN2A/p16INK4a antibody [EP435Y-129R] (ab81278)
This product has been referenced in:
Prabhavathy D et al. Re-expression of HPV16 E2 in SiHa (human cervical cancer) cells potentiates NF-?B activation induced by TNF-a concurrently increasing senescence and survival. Biosci Rep35:N/A (2015).
Read more (PubMed: 25572145) »
Xu H et al. Inherited coding variants at the CDKN2A locus influence susceptibility to acute lymphoblastic leukaemia in children. Nat Commun6:7553 (2015).
Read more (PubMed: 26104880) »