Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034)

Overview

  • Product name
    Anti-CDKN2A/p16INK4a antibody - N-terminal
    See all CDKN2A/p16INK4a primary antibodies
  • Description
    Rabbit polyclonal to CDKN2A/p16INK4a - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human CDKN2A/p16INK4a (N terminal). 19 amino acid peptide.
    Database link: P42771

  • Positive control
    • Human colon tissues. Human colon cells. Mouse colon tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab189034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Cellular localization
    Cytoplasmic and Nuclear
  • Database links
  • Form
    There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF.
  • Alternative names
    • CCM2 antibody
    • CDK4 inhibitor p16 INK4 antibody
    • CDK4I antibody
    • CDKN2 antibody
    • CDKN2A antibody
    • Cell cycle negative regulator beta antibody
    • CMM2 antibody
    • Cyclin dependent kinase 4 inhibitor A antibody
    • Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody
    • Cyclin Dependent Kinase Inhibitor 2A antibody
    • Cyclin dependent kinase inhibitor 2A isoform 4 antibody
    • Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody
    • Cyclin dependent kinase inhibitor p16 antibody
    • INK4 antibody
    • INK4A antibody
    • MLM antibody
    • MTS1 antibody
    • Multiple tumor suppressor 1 antibody
    • p14 antibody
    • p16 antibody
    • P16INK4 antibody
    • p16INK4a antibody
    • p19 antibody
    • p19Arf antibody
    • TP16 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CDKN2A/p16INK4a with ab189034 at 5 µg/ml. Heat mediated antigen retrieval was performed.

  • Lane 1 : Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) at 1 µg/ml
    Lane 2 : Anti-CDKN2A/p16INK4a antibody - N-terminal (ab189034) at 2 µg/ml

    All lanes : mouse colon tissue lysate
  • Immunofuorescent analysis of human colon cells labeling CDKN2A using ab189034 at 20 µg/ml.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human colon tissue labeling CDKN2A using ab189034 at 5 µg/ml.

References

This product has been referenced in:
  • Chen K  et al. Optineurin-mediated mitophagy protects renal tubular epithelial cells against accelerated senescence in diabetic nephropathy. Cell Death Dis 9:105 (2018). IF . Read more (PubMed: 29367621) »
  • Yan Z  et al. Curcumin exerts a protective effect against premature ovarian failure in mice. J Mol Endocrinol 60:261-271 (2018). Read more (PubMed: 29437881) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
50 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Archana Raman

Verified customer

Submitted Dec 08 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse chondrocytes)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
30 µg
Treatment
siRNA against CDKN2a
Specification
mouse chondrocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Aug 08 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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