Overview

  • Product name
    Anti-CDKN2A/p19ARF antibody [5-C3]
    See all CDKN2A/p19ARF primary antibodies
  • Description
    Rat monoclonal [5-C3] to CDKN2A/p19ARF
  • Tested applications
    Suitable for: WB, IP, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Hamster, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Mouse p19ARF.

  • Positive control
    • 3T3 cell lystate positive control.
  • General notes

    This antibody clone is manufactured by Abcam.

    Alternative versions available:

    Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free (ab174939)

Properties

Applications

Our Abpromise guarantee covers the use of ab26696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/1000.
IHC-FoFr Use a concentration of 5 µg/ml.

Target

  • Relevance
    The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • ARF antibody
    • CDKN2A antibody
    • Cyclin dependent kinase inhibitor 2A antibody
    • INK4 antibody
    • MTS1 antibody
    • P19 antibody
    see all

Anti-CDKN2A/p19ARF antibody [5-C3] images

  • All lanes : Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

    Lane 1 : Total cell lysate from NIH3T3 cells with Arf gene deleted
    Lane 2 : MEF total cell lysate

    Secondary
    anti-rat IgG-HRP

    Predicted band size : 17 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).
  • Immunoprecipitation using anti-p19ARF 5C3. NIH3T3 cells expressing HA-p19ARF were incubated with a control rat IgG (lane 1) or anti-p19ARF 5C3 (2). Precipitated proteins were analysed by Western blot using anti-HA antibody.

References for Anti-CDKN2A/p19ARF antibody [5-C3] (ab26696)

This product has been referenced in:
  • Bartesaghi S  et al. Inhibition of oxidative metabolism leads to p53 genetic inactivation and transformation in neural stem cells. Proc Natl Acad Sci U S A 112:1059-64 (2015). WB . Read more (PubMed: 25583481) »
  • Shai A  et al. TP53 Silencing Bypasses Growth Arrest of BRAFV600E-Induced Lung Tumor Cells in a Two-Switch Model of Lung Tumorigenesis. Cancer Res 75:3167-80 (2015). WB . Read more (PubMed: 26001956) »

See all 12 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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