Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free (ab174939)

Overview

  • Product name
    Anti-CDKN2A/p19ARF antibody [5-C3] - BSA and Azide free
    See all CDKN2A/p19ARF primary antibodies
  • Description
    Rat monoclonal [5-C3] to CDKN2A/p19ARF - BSA and Azide free
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Hamster, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • 3T3 cell lystate positive control.
  • General notes

    This antibody clone is manufactured by Abcam.

    This product is the PBS-only buffer version of ab26696

Properties

Applications

Our Abpromise guarantee covers the use of ab174939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • ARF antibody
    • CDKN2A antibody
    • Cyclin dependent kinase inhibitor 2A antibody
    • INK4 antibody
    • MTS1 antibody
    • P19 antibody
    see all

Images

  • This ICC/IF data was generated using the same antibody clone in a different buffer formulation (ab26696). 

    Immunofluorescent detection of p19ARF. MEF cells (1 and 2) or NIH3T3 cells with arf gene deleted (3 and 4) were stained with anti-p19ARF 5C3 followed by a fluorescent-labelled anti-rat IgG (1 and 3) and Hoechst dye to visualise nuclei (2 and 4).

References

ab174939 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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