This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Overview

Properties

Applications

  • Application notes
    This antibody gave a positive result in ELISA against the immunizing peptide (ab34188).
    Not yet tested in other applications.

  • Target

    • FunctionComponent of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. Promotes differentiation of myogenic cells.
    • Involvement in diseaseHoloprosencephaly 11
    • Sequence similaritiesContains 3 fibronectin type-III domains.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDO antibody
      • Cdon antibody
      • CDON_HUMAN antibody
      • Cell adhesion molecule-related/down-regulated by oncogenes antibody

    Anti-CDON / Cdo antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • ICC/IF image of ab34189 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab34189, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.  However, this Fast-Track antibody is not yet fully characterised.  This image represents inconclusive preliminary data.

    References for Anti-CDON / Cdo antibody (ab34189)

    ab34189 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (KM-H2)
    Specification KM-H2
    Preparation Cell harvesting/tissue preparation method: Asynchronous KM-H2 were harvested and labeled by indirect immunofluorescence
    Sample buffer: PBS + 5% FBS
    Permeabilization No
    Gating Strategy Forward/Side scatter were used to eliminate cellular debris. The accompanying marker was applied such that only 2% of the IgG control was positive.
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Dec 16 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"