Overview

  • Product name
  • Description
    Rabbit polyclonal to CDR2
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: MLAENLVEEF EMKEDEPWYD HQDLQQDLQL AAELGKTLLD RNTELEDSVQ, corresponding to N terminal amino acids 1-50 of Human CDR2

  • Positive control
    • HepG2 cell lysate and human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab65531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Sequence similarities
    Belongs to the CDR2 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA617262 antibody
    • CDR 2 antibody
    • CDR 62 antibody
    • CDR2 antibody
    • CDR2_HUMAN antibody
    • CDR62 antibody
    • Cerebellar degeneration related protein 2 62kDa antibody
    • Cerebellar degeneration related protein 2 antibody
    • Cerebellar degeneration related protein antibody
    • Cerebellar degeneration-related protein 2 antibody
    • Major Yo paraneoplastic antigen antibody
    • MGC116181 antibody
    • MGC144810 antibody
    • MGC144811 antibody
    • Paraneoplastic cerebellar degeneration associated antigen antibody
    • Paraneoplastic cerebellar degeneration-associated antigen antibody
    • PCD 17 antibody
    • PCD17 antibody
    • RGD1310578 antibody
    • Yo antibody
    • Yo paraneoplastic antigen antibody
    see all

Images

  • Anti-CDR2 antibody (ab65531) at 5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • ab65531 at 4µg/ml staining CDR2 in hepatocytes of human liver (indicated by arrows) by Immunohistochemistry, Paraffin-embedded tissue.

References

ab65531 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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