Overview

  • Product nameAnti-CDRL2 antibody
  • Description
    Rabbit polyclonal to CDRL2
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence corresponding to amino acids 395-464 (DSSWRDLRGG EEGQGEVKAG EKSLSQHVEA VDKRLEQSQP EYKALFKEIF SRIQKTKADI NATKVKTHSS) of Human CDRL2.

  • Positive control
    • RT-4, U-251 MG and Tonsil tissue lysate; Human cerebellum tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-CDRL2 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121705 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-CDRL2 antibody (ab121705) at 1/250 dilution

    Lane 1 : RT-4 cell lysate.
    Lane 2 : U-251 MG cell lysate.
    Lane 3 : Human Plasma
    Lane 4 : Human Liver tissue lysate.
    Lane 5 : Human Tonsil tissue lysate.

    Developed using the ECL technique
  • ab121705, at 1/125, staining CDRL2, in paraffin-embedded Human cerebellum tissue, by Immunohistochemistry.

References for Anti-CDRL2 antibody (ab121705)

ab121705 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"