Overview

  • Product name
    Anti-CDS1 antibody
  • Description
    Rabbit polyclonal to CDS1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (VVFGFIAAYV LSKYQYFVCP VEYRSDVNSF VTECEPSELF QLQTYSLPPF) of Human CDS1 (NP_001254).

  • Positive control
    • 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Provides CDP-diacylglycerol an important precursor for the synthesis of phosphatidylinositol (PtdIns), phosphatidylglycerol, and cardiolipin. Overexpression may amplify cellular signaling responses from cytokines. May also play an important role in the signal transduction mechanism of retina and neural cells.
  • Tissue specificity
    Expressed in adult tissues such as placenta, brain, small intestine, ovary, testis and prostate. Highly expressed in fetal kidney, lung and brain. Lower level in fetal liver.
  • Pathway
    Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.
  • Sequence similarities
    Belongs to the CDS family.
  • Cellular localization
    Endoplasmic reticulum membrane. Cytoplasmic aspect of the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDP DAG synthase 1 antibody
    • CDP DG synthetase 1 antibody
    • CDP diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 antibody
    • CDP diacylglycerol synthase 1 antibody
    • CDP diglyceride pyrophosphorylase 1 antibody
    • CDP diglyceride synthetase 1 antibody
    • CDP-DAG synthase 1 antibody
    • CDP-DG synthase 1 antibody
    • CDP-diacylglycerol synthase 1 antibody
    • CDP-diglyceride pyrophosphorylase 1 antibody
    • CDP-diglyceride synthase 1 antibody
    • CDS 1 antibody
    • CDS antibody
    • CDS1 antibody
    • CDS1_HUMAN antibody
    • CTP:phosphatidate cytidylyltransferase 1 antibody
    • Phosphatidate cytidylyltransferase 1 antibody
    see all

Images

  • Anti-CDS1 antibody (ab84019) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Croston TL  et al. Evaluation of the cardiolipin biosynthetic pathway and its interactions in the diabetic heart. Life Sci 93:313-22 (2013). Read more (PubMed: 23872101) »

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Rat Tissue lysate - other (Heart)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
50 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Evelyn Gomez Espinosa

Verified customer

Submitted Sep 04 2015

Thanks for your call today and I'm sorry that this antibody did not work as expected. I have issued a discount code, which will be activated by submitting an Abreview of ab84019 with rat samples. You canredeem this discount code for any primary antibod...

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Thank you again for your call yesterday, and also for your patience while I have looked through some literature as well as through the Western blot images.

I do have a couple of additional questions to clarify this data, as I haven't worked w...

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Thanks for your call today and for letting us know about the trouble with ab84019.

As we discussed, please forward me an image of your blot when you get a chance. I'll check the literature and our protocol for this antibody and see if there '...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 mouse fibroblast cell line)
Loading amount
40 µg
Specification
NIH 3T3 mouse fibroblast cell line
Treatment
48 hs-serum deprived
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. GERMAN GIL

Verified customer

Submitted Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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