Overview

  • Product nameAnti-CDT1 antibody
    See all CDT1 primary antibodies
  • Description
    Rabbit polyclonal to CDT1
  • Tested applicationsSuitable for: IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee
  • Immunogen

    A region between residue 500 and the C-terminus (residue 546) of human CDT1 (NP_112190.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/2500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

Target

  • FunctionCooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
  • Sequence similaritiesBelongs to the Cdt1 family.
  • Developmental stagePresent during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
  • DomainThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
  • Post-translational
    modifications
    Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, in response to DNA damage, leading to its degradation. Ubiquitination by the DCX(DTL) complex is necessary to ensure proper cell cycle regulation and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. The interaction with GMNN protects it against ubiquitination.
    Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDT 1 antibody
    • cdt1 antibody
    • CDT1_HUMAN antibody
    • Chromatin licensing and DNA replication factor 1 antibody
    • DNA replication factor antibody
    • DNA replication factor Cdt1 antibody
    • Double parked antibody
    • Double parked Drosophila homolog of antibody
    • Double parked homolog antibody
    • DUP antibody
    • Retroviral integration site 1 antibody
    • Retroviral integration site 2 antibody
    • Retroviral integration site1 antibody
    • Retroviral integration site2 antibody
    • RIS 2 antibody
    • RIS2 antibody
    see all

Anti-CDT1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human islet cell carcinoma tissue labelling CDT1 with ab70829 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-CDT1 antibody (ab70829) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa


    Exposure time : 3 minutes

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Detection of CDT1 by Western Blot of Immunprecipitate.
    ab70829 at 1µg/ml staining CDT1 in HeLa whole cell lysate immunoprecipitated using ab70829 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

    Secondary antibody anti-rabbit HRP (ab6721)
    Detection: Chemiluminescence with exposure time of 3 minutes.

References for Anti-CDT1 antibody (ab70829)

This product has been referenced in:
  • Hernández-Pérez S  et al. USP37 deubiquitinates Cdt1 and contributes to regulate DNA replication. Mol Oncol N/A:N/A (2016). WB ; Human . Read more (PubMed: 27296872) »
  • Clijsters L & Wolthuis R PIP-box-mediated degradation prohibits re-accumulation of Cdc6 during S phase. J Cell Sci 127:1336-45 (2014). Read more (PubMed: 24434580) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa S3 cervical cancer)
Loading amount 50 µg
Specification HeLa S3 cervical cancer
Treatment double thymidine block and release
Gel Running Conditions Reduced Denaturing (8% Tris-glycine SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SAOS-2 OSTEOSARCOMA)
Specification SAOS-2 OSTEOSARCOMA
Fixative Paraformaldehyde
Permeabilization Yes - PBS TRITON .25%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 03 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (embryonic stem cells)
Specification embryonic stem cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0.5%
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Mr. Shai Melcer

Verified customer

Submitted Nov 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Balb MK/ mouse keratinocyte / and 3T3 / and CHO)
Loading amount 500000 cells
Specification Balb MK/ mouse keratinocyte / and 3T3 / and CHO
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Mr. Thinh Cao

Verified customer

Submitted May 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"