Overview

  • Product name
  • Description
    Rabbit polyclonal to CDT2
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide to a region within: pssqtpnsrr qsgktlpspv titpssmrki ctyfhrksqe dfcgpehste l, corresponding to C terminal amino acids 680 - 730 of Human CDT2 (NP_057532.1)

  • Positive control
    • Human Breast Carcinoma, Colon Carcinoma and Stomach Adenocarcinoma Mouse Renal Cell Carcinoma and hybridoma tumor

Properties

Applications

Our Abpromise guarantee covers the use of ab95129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

Target

  • Function
    Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
  • Tissue specificity
    Expressed in placenta and testis, very low expression seen in skeletal muscle. Detected in all hematopoietic tissues examined, with highest expression in thymus and bone marrow. A low level detected in the spleen and lymph node, and barely detectable level in the peripheral leukocytes. RA treatment down-regulated the expression in NT2 cell.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the WD repeat cdt2 family.
    Contains 7 WD repeats.
  • Developmental stage
    Expressed in all fetal tissues examined, included brain, lung, liver, and kidney.
  • Post-translational
    modifications
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C).
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Nucleus membrane. Cytoplasm > cytoskeleton > centrosome. Nuclear matrix-associated protein. Translocates from the interphase nucleus to the metaphase cytoplasm during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lethal(2) denticleless protein homolog antibody
    • CDW1 antibody
    • DCAF2 antibody
    • DDB1 and CUL4 associated factor 2 antibody
    • Ddb1- and Cul4-associated factor 2 antibody
    • Denticleless homolog antibody
    • Denticleless homolog (Drosophila) antibody
    • Denticleless protein homolog antibody
    • Dtl antibody
    • DTL_HUMAN antibody
    • L2DTL antibody
    • Lethal(2) denticleless protein homolog antibody
    • RA regulated nuclear matrix associated protein antibody
    • RAMP antibody
    • Retinoic acid regulated nuclear matrix associated protein antibody
    • Retinoic acid-regulated nuclear matrix-associated protein antibody
    see all

Anti-CDT2 antibody images

  • ab95129, at 1/250 dilution, staining CDT2 in formalin-fixed, paraffin-embedded Human colon carcinoma by Immunohistochemistry, using DAB staining.
  • ab95129, at 1/250 dilution, staining CTD2 in formalin-fixed, paraffin-embedded Mouse hybridoma tumor by Immunohistochemistry, using DAB staining.

References for Anti-CDT2 antibody (ab95129)

ab95129 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (SW948 colon cancer)
Loading amount
25 µg
Specification
SW948 colon cancer
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 19°C
Username

Abcam user community

Verified customer

Submitted Feb 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (colon adenocarcinoma)
Specification
colon adenocarcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TEG
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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