The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/400.
(for 2 hours at RT)
Use 5-10µl for 106 cells. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
1/100 - 1/200.
(2 hours at RT)
1/50 - 1/100.
CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+ peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the costimulation of T-cells.