Overview

  • Product name
    Anti-CDw75 antibody [LN1]
    See all CDw75 primary antibodies
  • Description
    Mouse monoclonal [LN1] to CDw75
  • Host species
    Mouse
  • Specificity
    ab187273 reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin’s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CDw75. Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.
    Database link: P15907

  • Positive control
    • Human spleen tissue; Daudi Cells. Germinal center B-cells in a lymph node or tonsil. ab187273's ability to stain surface of erythrocytes cab be used as an internal positive control.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Clonality
    Monoclonal
  • Clone number
    LN1
  • Isotype
    IgM
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use 5-10µl for 106 cells.

ab18400 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

ICC/IF 1/25 - 1/50.

Target

  • Relevance
    CDw75 is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene.
  • Cellular localization
    Golgi Apparatus; Golgi stack; Golgi stack membrane; single-pass type II membrane protein. Membrane-bound form in trans cisternae of Golgi. Secreted protein; body fluid.
  • Database links
  • Alternative names
    • Alpha 2 6 ST antibody
    • B cell antigen CD75 antibody
    • Beta galactoside alpha 2 6 sialyltransferase antibody
    • CD75 antibody
    • CMP N acetylneuraminate beta galactosamide alpha 2 6 sialyltransferase antibody
    • MGC48859 antibody
    • Sialyltransferase 1 antibody
    • SIAT 1 antibody
    • SIAT1 antibody
    • ST6 beta galactosamide alpha 2 6 sialyltranferase 1 antibody
    • ST6GAL 1 antibody
    • ST6Gal I antibody
    • ST6GAL1 antibody
    • ST6GalI antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded normal Human spleen tissue labeling CDw75 using ab187273 at a 1/50 dilution.

  • Flow cytometric analysis of Human lymphocytes labeling CDw75 with ab187273 at 5 µl/10 6 cells (red) and isotype control (green). PPI negative population analyzed.

References

ab187273 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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