Recombinant
RabMAb

Anti-Cdx1 antibody [EPR4819(2)] (ab126748)

Overview

  • Product name
    Anti-Cdx1 antibody [EPR4819(2)]
    See all Cdx1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4819(2)] to Cdx1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • Caco-2 and Human stomach lysates; Caco-2 cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Could play a role in the terminal differentiation of the intestine.
    • Tissue specificity
      Intestinal epithelium.
    • Sequence similarities
      Belongs to the Caudal homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Caudal type homeo box transcription factor 1 antibody
      • Caudal type homeobox 1 antibody
      • Caudal type homeobox protein antibody
      • Caudal type homeobox protein CDX1 antibody
      • Caudal type homeobox transcription factor 1 antibody
      • Caudal-type homeobox protein 1 antibody
      • CDX1 antibody
      • CDX1_HUMAN antibody
      • Homeobox protein CDX 1 antibody
      • Homeobox protein CDX-1 antibody
      • MGC116915 antibody
      • OTTHUMP00000160524 antibody
      see all

    Images

    • All lanes : Anti-Cdx1 antibody [EPR4819(2)] (ab126748) at 1/1000 dilution

      Lane 1 : Caco-2 lysate
      Lane 2 : Human stomach lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 28 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    • ab126748, at 1/100 dilution, staining Cdx1 in Caco-2 cells by Immunofluorescence.

    References

    ab126748 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Differentiated human embryonic stem cells)
    Permeabilization
    Yes - PBS/0.1% Triton X
    Specification
    Differentiated human embryonic stem cells
    Blocking step
    1% BSA/3% Donkey serum in PBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 07 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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