Anti-CDX2 antibody (ab105505)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab105505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInvolved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
  • Sequence similaritiesBelongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-60 mediates the transactivation capacity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Caudal type homeo box 2 antibody
    • Caudal type homeo box transcription factor 2 antibody
    • Caudal type homeobox 2 antibody
    • Caudal type homeobox protein 2 antibody
    • Caudal type homeobox transcription factor 2 antibody
    • Caudal-type homeobox protein 2 antibody
    • CDX 2 antibody
    • CDX 3 antibody
    • CDX-3 antibody
    • Cdx2 antibody
    • CDX2_HUMAN antibody
    • CDX3 antibody
    • Homeobox protein CDX 2 antibody
    • Homeobox protein CDX-2 antibody
    see all

Anti-CDX2 antibody images

  • Anti-CDX2 antibody (ab105505) + Fetal Brain

    Predicted band size : 34 kDa

References for Anti-CDX2 antibody (ab105505)

ab105505 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (Blastocyst)
Permeabilization Yes - 1% Triton 100X in PBS
Specification Blastocyst
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Rodrigo Bohrer

Verified customer

Submitted Aug 03 2015

Thank you for your message. I have investigated the data for ab105505 further and agree that the band would not be expected at this molecular weight. My colleagues and our originator are currently investigating this and re testing of the antibody will ...

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I have not found any information as to whether HEK T cells may be used as a negative control. The following journal however suggests that the following colon cancer lines: HCT 116, HT-29, RKO, SW480 and WiDr may be suitable as negative controls...

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Thank you for contacting us. I am pleased to hear you are having positive results with our antibody. I have looked into whether there is a suitable negative control. I would not suggest using K562 as there are a alternative antibodies against CDX2 whic...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"