The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1-4 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionInvolved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Sequence similaritiesBelongs to the Caudal homeobox family. Contains 1 homeobox DNA-binding domain.
Post-translational modificationsPhosphorylation of Ser-60 mediates the transactivation capacity.