Anti-CDX2 antibody [CDX2-88], prediluted (ab86949)

Overview

  • Product nameAnti-CDX2 antibody [CDX2-88], prediluted
    See all CDX2 primary antibodies
  • Description
    Mouse monoclonal [CDX2-88] to CDX2, prediluted
  • Tested applicationsSuitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Dog, Human
  • Immunogen

    CDX2 protein (Human)

  • Positive control
    • Colon cancer Normal Tissue: Colon Abnormal Tissue: Colon cancer, cyst adenocarcinoma and mucinous ovarian cancer

Properties

Applications

Our Abpromise guarantee covers the use of ab86949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Primary Antibody: incubate for 30 minutes at RT.
Flow Cyt Use at an assay dependent concentration. Use Neat



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
  • Sequence similaritiesBelongs to the Caudal homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylation of Ser-60 mediates the transactivation capacity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Caudal type homeo box 2 antibody
    • Caudal type homeo box transcription factor 2 antibody
    • Caudal type homeobox 2 antibody
    • Caudal type homeobox protein 2 antibody
    • Caudal type homeobox transcription factor 2 antibody
    • Caudal-type homeobox protein 2 antibody
    • CDX 2 antibody
    • CDX 3 antibody
    • CDX-3 antibody
    • Cdx2 antibody
    • CDX2_HUMAN antibody
    • CDX3 antibody
    • Homeobox protein CDX 2 antibody
    • Homeobox protein CDX-2 antibody
    see all

Anti-CDX2 antibody [CDX2-88], prediluted images

  • Prediluted ab86949, staining CDX2 in formalin fixed, paraffin embedded Human colon cancer tissue by Immunohistochemistry.
  • Overlay histogram showing HCT116 cells stained with ab86949 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86949, neat) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-CDX2 antibody [CDX2-88], prediluted (ab86949)

This product has been referenced in:
  • Anani S  et al. Initiation of Hippo signaling is linked to polarity rather than to cell position in the pre-implantation mouse embryo. Development 141:2813-24 (2014). Read more (PubMed: 24948601) »
  • Hashimoto K  et al. Immunohistochemical profile for unknown primary adenocarcinoma. PLoS One 7:e31181 (2012). IHC-P ; Human . Read more (PubMed: 22299055) »

See all 2 Publications for this product

Product Wall


The flow data were obtained using 100ul neat antibody (undiluted) with 1x10^6 cells.

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