Recombinant
RabMAb

Anti-CDX2 antibody [EPR2764Y] (ab76541)

Overview

  • Product name
    Anti-CDX2 antibody [EPR2764Y]
    See all CDX2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2764Y] to CDX2
  • Specificity
    We have some successful data from mouse and rat samples in some submitted Abreviews and alignment of the immunogen with the mouse and rat proteins is high. However, please note that results in testing of mouse and rat have been inconsistent.
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CDX2 aa 1-100 (N terminal).

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

     

    Alternative versions available:

    Anti-CDX2 antibody (Alexa Fluor® 488) [EPR2764Y] (ab195007)
    Anti-CDX2 antibody (Alexa Fluor® 647) [EPR2764Y] (ab195008)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab76541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
    • Sequence similarities
      Belongs to the Caudal homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylation of Ser-60 mediates the transactivation capacity.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Caudal type homeo box 2 antibody
      • Caudal type homeo box transcription factor 2 antibody
      • Caudal type homeobox 2 antibody
      • Caudal type homeobox protein 2 antibody
      • Caudal type homeobox transcription factor 2 antibody
      • Caudal-type homeobox protein 2 antibody
      • CDX 2 antibody
      • CDX 3 antibody
      • CDX-3 antibody
      • Cdx2 antibody
      • CDX2/AS antibody
      • CDX2_HUMAN antibody
      • CDX3 antibody
      • Homeobox protein CDX 2 antibody
      • Homeobox protein CDX-2 antibody
      see all

    Anti-CDX2 antibody [EPR2764Y] images

    • Lane 1 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/1000 dilution
      Lanes 2 - 3 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/5000 dilution

      Lane 1 : HT29 lysate
      Lane 2 : Colon cancer lysate
      Lane 3 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    • ab76541 staining CDX2 in human colon cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500) for 2 hours. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • ab76541, at 1/250 dilution, staining CDX2 in paraffin-embedded human gastric adenocarcinoma tissue, by immunohistochemistry.
    • ab76541 staining CDX2 in Human intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in PBS containing 5% BSA) for 18 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • ab76541 showing positive staining in Colonic adenocarcinoma tissue.

    • ab76541 showing positive staining in Normal colon tissue.

    • ab76541 showing negative staining in Skeletal muscle tissue.

    • ab76541 showing negative staining in Normal kidney tissue.

    • ab76541 showing negative staining in Normal brain tissue.

    • ab76541 showing negative staining in Normal brain tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CDX2 antibody [EPR2764Y] (ab76541)

    This product has been referenced in:
    • Posfai E  et al. Position- and Hippo signaling-dependent plasticity during lineage segregation in the early mouse embryo. Elife 6:N/A (2017). Read more (PubMed: 28226240) »
    • Uchida H  et al. A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells. JCI Insight 2:e86492 (2017). Read more (PubMed: 28097227) »

    See all 19 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (differentiated human ES cells)
    Permeabilization
    Yes - PBS/0.1% Triton-X
    Specification
    differentiated human ES cells
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 16 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - other (Colon cancer lysate)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    5 µg
    Specification
    Colon cancer lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 08 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Peritoneal tumor)
    Permeabilization
    Yes - 0.3% Triton X100
    Specification
    Peritoneal tumor
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 24 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Apr 30 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Colon
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Apr 30 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Embryonic ( e14) intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Embryonic ( e14) intestine
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Apr 15 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Colon)
    Specification
    Colon
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 07 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Sample
    Rabbit Cell (Blastocyst)
    Specification
    Blastocyst
    Permeabilization
    Yes - Triton-X 100
    Fixative
    Formaldehyde
    Username

    Dr. Yolanda Markaki

    Verified customer

    Submitted Jun 28 2013

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Preimplantation embryo)
    Specification
    Preimplantation embryo
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.2% Triton X-100
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 25 2013

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More

    1-10 of 16 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up