Overview

  • Product nameAnti-CDX2 antibody [EPR2764Y]
    See all CDX2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2764Y] to CDX2
  • SpecificityWe have some successful data from mouse and rat samples in some submitted Abreviews and alignment of the immunogen with the mouse and rat proteins is high. However, please note that results in testing of mouse and rat have been inconsistent.
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CDX2 aa 1-100 (N terminal).

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-CDX2 antibody (Alexa Fluor® 488) [EPR2764Y] (ab195007)
    Anti-CDX2 antibody (Alexa Fluor® 647) [EPR2764Y] (ab195008)

Properties

Applications

Our Abpromise guarantee covers the use of ab76541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionInvolved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
    • Sequence similaritiesBelongs to the Caudal homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylation of Ser-60 mediates the transactivation capacity.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Caudal type homeo box 2 antibody
      • Caudal type homeo box transcription factor 2 antibody
      • Caudal type homeobox 2 antibody
      • Caudal type homeobox protein 2 antibody
      • Caudal type homeobox transcription factor 2 antibody
      • Caudal-type homeobox protein 2 antibody
      • CDX 2 antibody
      • CDX 3 antibody
      • CDX-3 antibody
      • Cdx2 antibody
      • CDX2_HUMAN antibody
      • CDX3 antibody
      • Homeobox protein CDX 2 antibody
      • Homeobox protein CDX-2 antibody
      see all

    Anti-CDX2 antibody [EPR2764Y] images

    • Lane 1 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/1000 dilution
      Lanes 2 - 3 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/5000 dilution

      Lane 1 : HT29 lysate
      Lane 2 : Colon cancer lysate
      Lane 3 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    • ab76541 staining CDX2 in human colon cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500) for 2 hours. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • ab76541, at 1/250 dilution, staining CDX2 in paraffin-embedded human gastric adenocarcinoma tissue, by immunohistochemistry.
    • ab76541 staining CDX2 in Human intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in PBS containing 5% BSA) for 18 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • ab76541 showing positive staining in Colonic adenocarcinoma tissue.

    • ab76541 showing positive staining in Normal colon tissue.

    • ab76541 showing negative staining in Skeletal muscle tissue.

    • ab76541 showing negative staining in Normal kidney tissue.

    • ab76541 showing negative staining in Normal brain tissue.

    • ab76541 showing negative staining in Normal brain tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CDX2 antibody [EPR2764Y] (ab76541)

    This product has been referenced in:
    • Yang Y  et al. Heightened potency of human pluripotent stem cell lines created by transient BMP4 exposure. Proc Natl Acad Sci U S A 112:E2337-46 (2015). Read more (PubMed: 25870291) »
    • Ohinata Y & Tsukiyama T Establishment of trophoblast stem cells under defined culture conditions in mice. PLoS One 9:e107308 (2014). ICC/IF . Read more (PubMed: 25203285) »

    See all 11 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - other (Colon cancer lysate)
    Gel Running Conditions Reduced Denaturing (10)
    Loading amount 5 µg
    Specification Colon cancer lysate
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 08 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Peritoneal tumor)
    Permeabilization Yes - 0.3% Triton X100
    Specification Peritoneal tumor
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 24 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Colon)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Colon
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Apr 30 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Colon)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Colon
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Apr 30 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Embryonic ( e14) intestine)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Embryonic ( e14) intestine
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Apr 15 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Colon)
    Specification Colon
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Feb 07 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Sample Rabbit Cell (Blastocyst)
    Specification Blastocyst
    Permeabilization Yes - Triton-X 100
    Fixative Formaldehyde
    Username

    Dr. Yolanda Markaki

    Verified customer

    Submitted Jun 28 2013

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Preimplantation embryo)
    Specification Preimplantation embryo
    Fixative Formaldehyde
    Permeabilization Yes - 0.2% Triton X-100
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 25 2013

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More

    Could you provide the purchase order number for us to proceed with order?

    Please note, if you have purchased the antibody from our distributor then I would suggest contacting them for discussion about agreed free of charge replacement.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"