Recombinant
RabMAb

Anti-CDX2 antibody [EPR2764Y] (ab76541)

Overview

  • Product name
    Anti-CDX2 antibody [EPR2764Y]
    See all CDX2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2764Y] to CDX2
  • Specificity
    We have some successful data from mouse and rat samples in some submitted Abreviews and alignment of the immunogen with the mouse and rat proteins is high. However, please note that results in testing of mouse and rat have been inconsistent.
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CDX2 aa 1-100 (N terminal).

  • Positive control
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

     

    Alternative versions available:

    Anti-CDX2 antibody (Alexa Fluor® 488) [EPR2764Y] (ab195007)
    Anti-CDX2 antibody (Alexa Fluor® 647) [EPR2764Y] (ab195008)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab76541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
    • Sequence similarities
      Belongs to the Caudal homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      modifications
      Phosphorylation of Ser-60 mediates the transactivation capacity.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Caudal type homeo box 2 antibody
      • Caudal type homeo box transcription factor 2 antibody
      • Caudal type homeobox 2 antibody
      • Caudal type homeobox protein 2 antibody
      • Caudal type homeobox transcription factor 2 antibody
      • Caudal-type homeobox protein 2 antibody
      • CDX 2 antibody
      • CDX 3 antibody
      • CDX-3 antibody
      • Cdx2 antibody
      • CDX2/AS antibody
      • CDX2_HUMAN antibody
      • CDX3 antibody
      • Homeobox protein CDX 2 antibody
      • Homeobox protein CDX-2 antibody
      see all

    Anti-CDX2 antibody [EPR2764Y] images

    • Lane 1 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/1000 dilution
      Lanes 2 - 3 : Anti-CDX2 antibody [EPR2764Y] (ab76541) at 1/5000 dilution

      Lane 1 : HT29 lysate
      Lane 2 : Colon cancer lysate
      Lane 3 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    • ab76541 staining CDX2 in human colon cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500) for 2 hours. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

      See Abreview

    • ab76541, at 1/250 dilution, staining CDX2 in paraffin-embedded human gastric adenocarcinoma tissue, by immunohistochemistry.
    • ab76541 staining CDX2 in Human intestinal tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hours at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100 in PBS containing 5% BSA) for 18 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

      See Abreview

    • ab76541 showing positive staining in Colonic adenocarcinoma tissue.

    • ab76541 showing positive staining in Normal colon tissue.

    • ab76541 showing negative staining in Skeletal muscle tissue.

    • ab76541 showing negative staining in Normal kidney tissue.

    • ab76541 showing negative staining in Normal brain tissue.

    • ab76541 showing negative staining in Normal brain tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CDX2 antibody [EPR2764Y] (ab76541)

    This product has been referenced in:
    • Posfai E  et al. Position- and Hippo signaling-dependent plasticity during lineage segregation in the early mouse embryo. Elife 6:N/A (2017). Read more (PubMed: 28226240) »
    • Uchida H  et al. A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells. JCI Insight 2:e86492 (2017). Read more (PubMed: 28097227) »

    See all 19 Publications for this product

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    Thank you for contacting us.

    ab76541 is an unconjugated primary antibody.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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