The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Antigen retrieval is recommended: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Use at an assay dependent concentration. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Sequence similaritiesBelongs to the Caudal homeobox family. Contains 1 homeobox DNA-binding domain.
Post-translational modificationsPhosphorylation of Ser-60 mediates the transactivation capacity.
Staining of Human CDX2 in a Formalin/PFA-fixed paraffin-embedded section of Human Colon Carcinoma using ab101532 at a dilution of 1/100.
References for Anti-CDX2 antibody [SP54] (ab101532)
This product has been referenced in:
Chen Y et al. 5-Aza-dC treatment induces mesenchymal-to-epithelial transition in 1st trimester trophoblast cell line HTR8/SVneo. Biochem Biophys Res Commun432:116-22 (2013).
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