Synthetic peptide derived from residues 300 - 400 of Mouse CDYL.
Our Abpromise guarantee covers the use of ab5188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|CHIPseq||Use at an assay dependent concentration. PubMed: 22009739|
|ChIP||Use at an assay dependent concentration. PubMed: 22009739|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 66 kDa).|
Cecile Lestrat, Saadi Khochbin lab, Grenoble, France
Image courtesy of Human Protein Atlas
ab5188 staining CDYL in human male kidney, showing a distinct and strong staining pattern in tubuli and glomeruli cells. Paraffin embedded human skin tissue was incubated with ab5188 (1/1000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab5188 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org