Overview

  • Product nameAnti-CEACAM1 antibody [GM8G5]
    See all CEACAM1 primary antibodies
  • Description
    Mouse monoclonal [GM8G5] to CEACAM1
  • Specificityab26279 recognises CEACAM1 transiently expressed on the cell surface of transfected BOSC cells as well as the naturally expressed protein. As the A2 domain is unique to CEACAM1, ab26279 can be used to distinguish CEACAM1 from all other CEACAM.
  • Tested applicationsSuitable for: Flow Cyt, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human CEACAM1-A2 domain cDNA (see relevance text).

  • General notes


    ab26279 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the cDNA of the human CEACAM1-A2 domain.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesab26279 was generated by genetic immunisation of BALB/c mice with an immunisation vector containing the cDNA of the human CEACAM1-A2 domain.
  • ClonalityMonoclonal
  • Clone numberGM8G5
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
  • Information by UniProt
  • Database links
  • FormThere are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S.
  • Alternative names
    • Antigen CD66 antibody
    • BGP 1 antibody
    • BGP antibody
    • BGP-1 antibody
    • BGPI antibody
    • Biliary glycoprotein 1 antibody
    • Biliary glycoprotein adhesion molecule antibody
    • Biliary glycoprotein antibody
    • Carcinoembryonic antigen related cell adhesion molecule 1 antibody
    • carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 1 antibody
    • CD66a antibody
    • CD66a antigen antibody
    • CEACAM1 antibody
    • CEAM1_HUMAN antibody
    • meconium antigen 100 antibody
    see all

Anti-CEACAM1 antibody [GM8G5] images

  • Immunohistological staining of colon tissue with ab26279. Cryosections of colon tissue were stained with 10 ug/ml ab26279 diluted in PBS containing 2.5% horse serum (B). PBS 2.5% horse serum served as negative control (A). Arrows indicate representative sections of analysed tissues. Detection of ab26279 occurred with a biotinylated anti-mouse-IgG secondary antibody and a streptavidin-peroxidase conjugate. Diaminobenzidine was used as substrate. Nuclei were stained with hematoxilin.

References for Anti-CEACAM1 antibody [GM8G5] (ab26279)

This product has been referenced in:
  • Thomas SN  et al. Carcinoembryonic Antigen and CD44 Variant Isoforms Cooperate to Mediate Colon Carcinoma Cell Adhesion to E- and L-selectin in Shear Flow. J Biol Chem 283:15647-55 (2008). Flow Cyt ; Human . Read more (PubMed: 18375392) »
  • Beaslas O  et al. TRANSCRIPTOME RESPONSE OF ENTEROCYTES TO DIETARY LIPIDS: IMPACT ON CELL ARCHITECTURE, SIGNALING AND METABOLISM GENES. Am J Physiol Gastrointest Liver Physiol : (2008). Read more (PubMed: 18755805) »

See all 2 Publications for this product

Product Wall

Sorry! please find attached now.

Thank you for contacting us. Please find attached the datasheet of ab26279. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Application Flow Cytometry
Sample Human Cell (Human foetal foreskin fibroblasts (HFFF))
Specification Human foetal foreskin fibroblasts (HFFF)
Permeabilization No
Username

Dr. Virginie Prod'Homme

Verified customer

Submitted Oct 05 2006

Thank you for your enquiry. The affinity of this antibody for CEACAM1 has not been tested, therefore I cannot answer your question. We do not have Fab2 or ScFv fragments of the antibody. If you cannot find what you are looking for within the Abc...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"