Overview

  • Product nameAnti-CEACAM19 antibody [HY-8H10]
    See all CEACAM19 primary antibodies
  • Description
    Mouse monoclonal [HY-8H10] to CEACAM19
  • SpecificityThis antibody reacts with CEACAM19.
  • Tested applicationsSuitable for: Flow Cyt, Competitive ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Genetic immunisation with cDNA encoding human CEACAM19.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberHY-8H10
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Competitive ELISA
  • Application notescELISA: 1/200 - 1/400.
    Flow Cyt: Use 1.2µg for 106 cells.
    SDS-PAGE: Use 2µg.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCEACAM19 belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. Like all members of the CEACAM family, the CEACAM19 protein is a single-pass type I membrane protein and consists of a single extracellular N domain. CEACAM19 expression is ubiquitous with highest expression in prostate, uterus, fetal brain, mammary gland, adrenal gland, skeletal muscle, small intestine, and kidney.
    • Cellular localizationCell Membrane; Single-pass type I membrane protein .
    • Database links
    • Alternative names
      • Carcinoembryonic antigen like 1 antibody
      • Carcinoembryonic antigen related cell adhesion molecule 19 antibody
      • CEA related cell adhesion molecule 19 antibody
      • CEAL1 antibody
      • MGC105097 antibody
      see all

    Anti-CEACAM19 antibody [HY-8H10] images

    • BOSC23 cells were transiently trans-fected with an expression vector encoding either CEACAM19 (red curve) or an irrelevant protein (control transfectant). Binding of ab59717 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM19 transfected cells.
    • Spectral Confocal Microscopy of CHO cells using ab59717.

      CHO cells were transiently transfected with an expression vector encoding CEACAM19. Binding of ab59717 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

    References for Anti-CEACAM19 antibody [HY-8H10] (ab59717)

    This product has been referenced in:
    • Kobayashi M  et al. Carcinoembryonic antigen-related cell adhesion molecules as surrogate markers for EGFR inhibitor sensitivity in human lung adenocarcinoma. Br J Cancer 107:1745-53 (2012). Read more (PubMed: 23099808) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab59717.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"