Overview

  • Product nameAnti-CEACAM6 antibody [9A6]
    See all CEACAM6 primary antibodies
  • Description
    Mouse monoclonal [9A6] to CEACAM6
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CEACAM6.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab78029 is purified.
  • ClonalityMonoclonal
  • Clone number9A6
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
IHC-P 1/40.

1/40 for 20 minutes at room temperature.

IHC-Fr 1/40.

1/40 for 20 minutes at room temperature.

Target

  • Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Carcinoembryonic antigen related cell adhesion molecule 6 antibody
    • Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) antibody
    • Carcinoembryonic antigen-related cell adhesion molecule 6 antibody
    • CD 66c antibody
    • CD66c antibody
    • CD66c antigen antibody
    • CEA LIKE PROTEIN antibody
    • CEACAM 6 antibody
    • CEACAM6 antibody
    • CEAL antibody
    • CEAM6_HUMAN antibody
    • MGC93832 antibody
    • NCA antibody
    • Non specific cross reacting antigen antibody
    • Non-specific crossreacting antigen antibody
    • Normal cross reacting antigen antibody
    • Normal cross-reacting antigen antibody
    see all

Anti-CEACAM6 antibody [9A6] images

  • ab78029 staining CEACAM6 in human breast carcinoma tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol and blocked with 10% serum for 5 minutes. Samples were incubated with primary antibody (1/200 in PBS + 10% goat serum) for 1 hour. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab78029 staining CEACAM6 in human cultured breast cancer cells by Immunocytochemistry. Cells were fixed with methanol and blocked with 10% serum for 5 minutes. Samples were incubated with the undiluted primary antibody for 1 hour. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-CEACAM6 antibody [9A6] (ab78029)

This product has been referenced in:
  • Zhang Y  et al. CEACAM6 promotes tumor migration, invasion, and metastasis in gastric cancer. Acta Biochim Biophys Sin (Shanghai) 46:283-90 (2014). Read more (PubMed: 24492534) »
  • Jarry A  et al. Subversion of human intestinal mucosa innate immunity by a Crohn's disease-associated E. coli. Mucosal Immunol N/A:N/A (2014). IHC ; Human . Read more (PubMed: 25269707) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10%
Sample Human Tissue sections (Breast carcinoma)
Specification Breast carcinoma
Permeabilization No
Fixative Methanol
Username

Dr. Rene Villadsen

Verified customer

Submitted Sep 19 2014

Application Immunocytochemistry
Sample Human Cultured Cells (Breast cancer cell line)
Specification Breast cancer cell line
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10%
Fixative Methanol
Username

Dr. Rene Villadsen

Verified customer

Submitted Sep 19 2014

Thank you for contacting us.
The batch-to-batch differences for monoclonal antibodies are not so significant as for polyclonal but slight variations can still occur due to manufacturing factors.
I can confirm that GR23152-2 was made at 115 ug...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"