Overview

  • Product nameAnti-CEBP Alpha antibody [EP708Y]
    See all CEBP Alpha primary antibodies
  • Description
    Rabbit monoclonal [EP708Y] to CEBP Alpha
  • SpecificityThis antibody recognizes both the 42kDa and the 30kDa isoforms.
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the N-term of human C/EBP alpha was used as immunogen.

  • Positive control
    • U937+LPS cell lysate, HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Anti-CEBP Alpha antibody (HRP) [EP708Y] (ab202678)

Properties

Applications

Our Abpromise guarantee covers the use of ab40761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 30,43 kDa (predicted molecular weight: 30,43 kDa).
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IHC or IP.
  • Target

    • FunctionC/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
    • Sequence similaritiesBelongs to the bZIP family. C/EBP subfamily.
      Contains 1 bZIP domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch 5 antibody
      • C/EBP alpha antibody
      • C/ebpalpha antibody
      • CAP4 antibody
      • Caspase 8 precursor antibody
      • CBF-A antibody
      • CCAAT Enhancer Binding Protein alpha antibody
      • CCAAT/enhancer binding protein (C/EBP), alpha antibody
      • CCAAT/enhancer-binding protein alpha antibody
      • CEBP antibody
      • CEBP A antibody
      • CEBP alpha antibody
      • Cebpa antibody
      • CEBPA_HUMAN antibody
      • FADD homologous ICE/CED 3 like protease antibody
      • FADD like ICE antibody
      • FLICE antibody
      • ICE like apoptotic protease 5 antibody
      • ICE8 antibody
      • MACH antibody
      • MCH5 antibody
      • MORT1 associated CED 3 homolog antibody
      see all

    Anti-CEBP Alpha antibody [EP708Y] images

    • Anti-CEBP Alpha antibody [EP708Y] (ab40761) at 1/1000 dilution + U937 + LPS cell lysate at 10 µg

      Predicted band size : 30,43 kDa
      Observed band size : 30,43 kDa
    • ab40761 (1/250) staining HeLa cells using immunofluorescence.
    • Overlay histogram showing HeLa cells stained with ab40761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Anti-CEBP Alpha antibody [EP708Y] (ab40761) at 1/5000 dilution + Human MCF-7 whole cell lysate at 100000 cells

      Secondary
      HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 30,43 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      See Abreview

    References for Anti-CEBP Alpha antibody [EP708Y] (ab40761)

    This product has been referenced in:
    • Federzoni EA  et al. CEBPA-dependent HK3 and KLF5 expression in primary AML and during AML differentiation. Sci Rep 4:4261 (2014). WB ; Human . Read more (PubMed: 24584857) »
    • Haefliger S  et al. Protein disulfide isomerase blocks CEBPA translation and is up-regulated during the unfolded protein response in AML. Blood 117:5931-40 (2011). WB ; Human . Read more (PubMed: 21471526) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MCF7)
    Loading amount 100000 cells
    Specification MCF7
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted May 11 2012

    Thank you for contacting Abcam.

    We do not currently have an image of how ab40761 works in western blot on HeLa cells, all of the western blot data we have for this antibody is on our website. The antibody is covered under our Abpromise for si...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HT1080 Cell line)
    Loading amount 200000 cells
    Specification HT1080 Cell line
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 04 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"