Overlay histogram showing HeLa cells stained with ab40764 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40764, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Western blot - Anti-CEBP Alpha antibody [EP709Y] (ab40764)This image is courtesy of an anonymous abreview.
Anti-CEBP Alpha antibody [EP709Y] (ab40764) at 1/1000 dilution + Human ESC (HUES7) whole cell lysate at 10 µg with 5% milk for 30 minutes at RT.
Secondary Monoclonal Donkey Anti-rabbit IgG HRP at 1/10000 dilution
References for Anti-CEBP Alpha antibody [EP709Y] (ab40764)
This product has been referenced in:
Weston W et al. Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets. Sci Rep4:5199 (2014).
Read more (PubMed: 24903657) »
Marzook H et al. Metastasis-associated protein 1 drives tumor cell migration and invasion through transcriptional repression of RING finger protein 144A. J Biol Chem287:5615-26 (2012).
WB, EMSA, IP
Read more (PubMed: 22184113) »