Overlay histogram showing HeLa cells stained with ab40764 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40764, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Western blot - Anti-CEBP Alpha antibody [EP709Y] (ab40764)This image is courtesy of an anonymous abreview.
Anti-CEBP Alpha antibody [EP709Y] (ab40764) at 1/1000 dilution + Human ESC (HUES7) whole cell lysate at 10 µg with 5% milk for 30 minutes at RT.
Secondary Monoclonal Donkey Anti-rabbit IgG HRP at 1/10000 dilution
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