Recombinant
RabMAb

Anti-CEBP Alpha antibody [EP709Y] (ab40764)

Overview

  • Product name
    Anti-CEBP Alpha antibody [EP709Y]
    See all CEBP Alpha primary antibodies
  • Description
    Rabbit monoclonal [EP709Y] to CEBP Alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: EMSA, WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-term of human CEBP Alpha

  • Positive control
    • U937 cell lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab40764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration. PubMed: 22184113
WB 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC.
  • Target

    • Function
      C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
    • Sequence similarities
      Belongs to the bZIP family. C/EBP subfamily.
      Contains 1 bZIP domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch 5 antibody
      • C/EBP alpha antibody
      • C/ebpalpha antibody
      • CAP4 antibody
      • Caspase 8 precursor antibody
      • CBF-A antibody
      • CCAAT Enhancer Binding Protein alpha antibody
      • CCAAT/enhancer binding protein (C/EBP), alpha antibody
      • CCAAT/enhancer-binding protein alpha antibody
      • CEBP antibody
      • CEBP A antibody
      • CEBP alpha antibody
      • Cebpa antibody
      • CEBPA_HUMAN antibody
      • FADD homologous ICE/CED 3 like protease antibody
      • FADD like ICE antibody
      • FLICE antibody
      • ICE like apoptotic protease 5 antibody
      • ICE8 antibody
      • MACH antibody
      • MCH5 antibody
      • MORT1 associated CED 3 homolog antibody
      see all

    Images

    • Anti-CEBP Alpha antibody [EP709Y] (ab40764) at 1/20000 dilution + U937 cell lysate at 10 µg

      Developed using the ECL technique.

      Performed under non-reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 43 kDa


      Exposure time: 12 seconds
    • Anti-CEBP Alpha antibody [EP709Y] (ab40764) at 1/1000 dilution + Human ESC (HUES7) whole cell lysate at 10 µg with 5% milk for 30 minutes at RT.

      Secondary
      Monoclonal Donkey Anti-rabbit IgG HRP at 1/10000 dilution

      Predicted band size: 43 kDa

      See Abreview

    References

    This product has been referenced in:
    • Sun Y  et al. Arctigenin Inhibits Liver Cancer Tumorigenesis by Inhibiting Gankyrin Expression via C/EBPa and PPARa. Front Pharmacol 9:268 (2018). Read more (PubMed: 29636686) »
    • Rodrigues T  et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . Read more (PubMed: 28490763) »

    See all 9 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (fat)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    20 µg
    Specification
    fat
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 14 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (ESC (HUES7))
    Gel Running Conditions
    Non-reduced Denaturing (12% gel)
    Loading amount
    10 µg
    Specification
    ESC (HUES7)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 09 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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