Anti-CEBP Beta antibody [1H7] - ChIP Grade (ab15050)

Overview

  • Product nameAnti-CEBP Beta antibody [1H7] - ChIP Grade
    See all CEBP Beta primary antibodies
  • Description
    Mouse monoclonal [1H7] to CEBP Beta - ChIP Grade
  • Tested applicationsSuitable for: ChIP, ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length LIP protein (Mouse)

  • Positive control
    • ICC/IF: P388D1 cells WB: 3T3-L1 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab15050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
ICC/IF Use a concentration of 4 µg/ml. Fix with 4% paraformaldehyde.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35,38,20 kDa).

Target

  • FunctionImportant transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
  • Tissue specificityExpressed at low levels in the lung, kidney and spleen.
  • Sequence similaritiesBelongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications
    Sumoylated by polymeric chains of SUMO2 or SUMO3.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGP/EBP antibody
    • C EBP beta antibody
    • C/EBP beta antibody
    • C/EBP related protein 2 antibody
    • CCAAT Enhancer Binding Protein beta antibody
    • CCAAT/enhancer-binding protein beta antibody
    • CEBPB antibody
    • CEBPB_HUMAN antibody
    • CRP2 antibody
    • IL 6DBP antibody
    • IL6DBP antibody
    • Interleukin 6 dependent binding protein antibody
    • LAP antibody
    • Liver activator protein antibody
    • Liver enriched transcriptional activator antibody
    • NF IL6 antibody
    • NFIL6 antibody
    • Nuclear factor NF IL6 antibody
    • Nuclear factor NF-IL6 antibody
    • SF B antibody
    • SFB antibody
    • Silencer factor B antibody
    • TCF-5 antibody
    • TCF5 antibody
    • Transcription factor 5 antibody
    see all

Anti-CEBP Beta antibody [1H7] - ChIP Grade images



  • Predicted band size : 35,38,20 kDa

    Western blot of the 3T3-L1 cell lysate using ab15050.

  • Immunofluorescence staining of CEBP beta in P388D1 cells using ab15050.

References for Anti-CEBP Beta antibody [1H7] - ChIP Grade (ab15050)

This product has been referenced in:
  • Liu H  et al. GCN2-dependent metabolic stress is essential for endotoxemic cytokine induction and pathology. Mol Cell Biol 34:428-38 (2014). Mouse . Read more (PubMed: 24248597) »
  • Mariani O  et al. JUN oncogene amplification and overexpression block adipocytic differentiation in highly aggressive sarcomas. Cancer Cell 11:361-74 (2007). Read more (PubMed: 17418412) »

See all 3 Publications for this product

Product Wall

Thank you for your reply.

We have not tested ab15050 in ChIP, therefore we cannot guarantee that it will work in this application. Since it does work for IP though, there is a good chance that it will be successful.

Please let me kn...

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Thank you for getting back to me on this.

I have put some red arrows next to what I believe are the correct size bands. It looks to me as though the correct bands are present in the mouse cell lysate (although faint) and that in your samples ...

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Thank you for confirming the address.

I have sent the vial of ab14632 to you and you should hopefully receive it on Thursday of this week.

Please let me know how you get on with this and whether it helps clarify the results that you...

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Thank you for contacting Abcam.

I have looked at the image you sent me and you are getting the correct banding pattern, but everything is 10-15kDa higher than it should be. So you could be really unlucky and the antibody is binding non-specif...

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Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"