Overview

  • Product nameAnti-CEBP Beta antibody [E299]
    See all CEBP Beta primary antibodies
  • Description
    Rabbit monoclonal [E299] to CEBP Beta
  • SpecificityThis antibody is specific for the three CEBPB isoforms (LAP*, LAP and LIP). According to BLAST analysis, the antibody could cross-react with CEBP epsilon (32, 27 and 14kDa, 82% homology) and CEBP alpha (42kDa, 30kDa, 73% homology) in human, mouse and rat. Please be aware that this has not been confirmed experimentally. However, this could explain the background that could possibly be obtained in WB with this antibody. Please contact our Scientific Support if you have any questions.
  • Tested applicationsSuitable for: EMSA, WB, ICC/IF, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C terminus of Rat CEBPB.

  • Positive control
    • WB: PC12 cell lysate. IF: Hela cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab32358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA Use at an assay dependent concentration. PubMed: 18462018
WB 1/1000. Predicted molecular weight: 36 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/20.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionImportant transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
    • Tissue specificityExpressed at low levels in the lung, kidney and spleen.
    • Sequence similaritiesBelongs to the bZIP family. C/EBP subfamily.
      Contains 1 bZIP domain.
    • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
    • Post-translational
      modifications
      Sumoylated by polymeric chains of SUMO2 or SUMO3.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AGP/EBP antibody
      • C EBP beta antibody
      • C/EBP beta antibody
      • C/EBP related protein 2 antibody
      • CCAAT Enhancer Binding Protein beta antibody
      • CCAAT/enhancer-binding protein beta antibody
      • CEBPB antibody
      • CEBPB_HUMAN antibody
      • CRP2 antibody
      • IL 6DBP antibody
      • IL6DBP antibody
      • Interleukin 6 dependent binding protein antibody
      • LAP antibody
      • Liver activator protein antibody
      • Liver enriched transcriptional activator antibody
      • NF IL6 antibody
      • NFIL6 antibody
      • Nuclear factor NF IL6 antibody
      • Nuclear factor NF-IL6 antibody
      • SF B antibody
      • SFB antibody
      • Silencer factor B antibody
      • TCF-5 antibody
      • TCF5 antibody
      • Transcription factor 5 antibody
      see all

    Anti-CEBP Beta antibody [E299] images

    • Anti-CEBP Beta antibody [E299] (ab32358) at 1/1000 dilution + PC12 cell lysate.

      Predicted band size : 36 kDa
      Observed bands
      LAP*: 38kDa
      LAP: 35kDa
      LIP: 20kDa
    • Ab32358, at a 1/50 dilution, staining CEBPB in Hela cells by Immunofluorescence.
    • Overlay histogram showing HeLa cells stained with ab32358 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32358, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-CEBP Beta antibody [E299] (ab32358) at 1/500 dilution

      Lane 1 : 3T3 Cells
      Lane 2 : MCF7 Cells
      Lane 3 : PC12 Cells
      Lane 4 : Rat Spleen Lysate
      Lane 5 : Rat Kidney Lysate
      Lane 6 : Rat Heart Lysate
      Lane 7 : Rat Brain Lysate
      Lane 8 : Mouse Spleen Lysate
      Lane 9 : Mouse Kidney Lysate
      Lane 10 : Mouse Heart Lysate
      Lane 11 : Mouse Brain Lysate


      Predicted band size : 36 kDa

    References for Anti-CEBP Beta antibody [E299] (ab32358)

    This product has been referenced in:
    • Guaraldo M  et al. Characterization of human mitochondrial ferritin promoter: identification of transcription factors and evidences of epigenetic control. Sci Rep 6:33432 (2016). ChIP ; Human . Read more (PubMed: 27625068) »
    • Viart V  et al. Transcription factors and miRNAs that regulate fetal to adult CFTR expression change are new targets for cystic fibrosis. Eur Respir J 45:116-28 (2015). Read more (PubMed: 25186262) »

    See all 9 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Non-reduced Denaturing (10% gel)
    Sample Mouse Cell lysate - whole cell (C3H10T cells)
    Specification C3H10T cells
    Treatment 25ng/ml (BMP4)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 26 2015

    Vielen Dank für Ihre Email.

    Ich habe mich an das Labor gewandt und habe nun eine Rückmeldung erhalten. Der Antikörper wurde im Labor für IHC und FC getestet und die erhaltenen Ergebnisse waren negativ. Das Labor schlie&szli...

    Read More

    Thank you for your inquiry. I am happy to confirm that when you are submitting the Abreview, you can choose to show your name and other scientists will also be able to contact you if you choose to permit this.

    DISCOUNT CODE: xxxxxx

    E...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (mcf7)
    Loading amount 100000 cells
    Specification mcf7
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted May 10 2012

    Thank you for contacting us.

    Yes, that would be lovely. As always, we would need the customers email and the institution he/she is working for.
    Looking forward to hearing from you.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - other (HT1080 Cell line)
    Loading amount 100000 cells
    Specification HT1080 Cell line
    Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Neil Taylor

    Verified customer

    Submitted Feb 08 2010

    Application Immunocytochemistry
    Sample Mouse Cultured Cells (Mouse Embryonic Fibroblasts)
    Specification Mouse Embryonic Fibroblasts
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton X-100
    Username

    Dr. Neil Taylor

    Verified customer

    Submitted Oct 06 2009

    Application Immunocytochemistry
    Sample Human Cultured Cells (HT1080 Cell line)
    Specification HT1080 Cell line
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% Triton X-100
    Username

    Dr. Neil Taylor

    Verified customer

    Submitted Oct 06 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"