Overview

  • Product nameAnti-CEBP Beta (phospho T235 + T188) antibody
    See all CEBP Beta primary antibodies
  • Description
    Rabbit polyclonal to CEBP Beta (phospho T235 + T188)
  • SpecificityThis antibody detects endogenous levels of CEBP Beta only when phosphorylated at threonine 235/188.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human CEBP Beta around the phosphorylation site of threonine 235/188.

  • Positive control
    • Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab52194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB Use at an assay dependent concentration. PubMed: 22144112

Target

  • FunctionImportant transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation.
  • Tissue specificityExpressed at low levels in the lung, kidney and spleen.
  • Sequence similaritiesBelongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Domainthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Post-translational
    modifications
    Sumoylated by polymeric chains of SUMO2 or SUMO3.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGP/EBP antibody
    • C EBP beta antibody
    • C/EBP beta antibody
    • C/EBP related protein 2 antibody
    • CCAAT Enhancer Binding Protein beta antibody
    • CCAAT/enhancer-binding protein beta antibody
    • CEBPB antibody
    • CEBPB_HUMAN antibody
    • CRP2 antibody
    • IL 6DBP antibody
    • IL6DBP antibody
    • Interleukin 6 dependent binding protein antibody
    • LAP antibody
    • Liver activator protein antibody
    • Liver enriched transcriptional activator antibody
    • NF IL6 antibody
    • NFIL6 antibody
    • Nuclear factor NF IL6 antibody
    • Nuclear factor NF-IL6 antibody
    • SF B antibody
    • SFB antibody
    • Silencer factor B antibody
    • TCF-5 antibody
    • TCF5 antibody
    • Transcription factor 5 antibody
    see all

Anti-CEBP Beta (phospho T235 + T188) antibody images

  • ab52194 staining CEBP Beta (phospho T335 + T188) in Human MDA-MB-468 breast cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde + 2% sucrose and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (in PBS + 1% BSA) for 30 minutes at 37°C.

    From left - DAPI (blue), MUC1-C (green), CERP Beta phospho T335 + T188 (red), Merge, Zoom.

    Top - CshRNA, Bottom - MUC1shRNA.

    Magnification - x63, Zoom (from pink boxes) - x252.

  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab52194 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with phosphopeptide.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab52194 at a 1/50 dilution.

    Left image un-treated.

    Right image treated with phosphopeptide.

References for Anti-CEBP Beta (phospho T235 + T188) antibody (ab52194)

This product has been referenced in:
  • Alam M  et al. MUC1-C Oncoprotein Activates ERK->C/EBPß Signaling and Induction of Aldehyde Dehydrogenase 1A1 in Breast Cancer Cells. J Biol Chem 288:30892-903 (2013). ICC/IF ; Human . Read more (PubMed: 24043631) »
  • Chen CJ  et al. Glutamate released by Japanese encephalitis virus-infected microglia involves TNF-a signaling and contributes to neuronal death. Glia 60:487-501 (2012). WB . Read more (PubMed: 22144112) »

See all 3 Publications for this product

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