Overview

  • Product name
  • Description
    Rabbit polyclonal to CEBP Delta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Sheep, Cow, Pig, Chimpanzee, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CEBP Delta aa 150-250 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P49716

  • Positive control
    • This antibody gave a positive signal in both Human and Rat Heart tissue lysates. ICC/IF: RAW246.7 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab155073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

Target

  • Function
    C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.
  • Sequence similarities
    Belongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C/EBP delta antibody
    • C/EBP related protein 3 antibody
    • CCAAT/enhancer binding protein (C/EBP) delta antibody
    • CCAAT/enhancer binding protein delta antibody
    • CCAAT/enhancer-binding protein delta antibody
    • CEBP D antibody
    • CEBPD antibody
    • CEBPD_HUMAN antibody
    • CELF antibody
    • Crp 3 antibody
    • Crp3 antibody
    • NF IL6 beta antibody
    • NF-IL6-beta antibody
    • Nuclear factor NF IL6 beta antibody
    • Nuclear factor NF-IL6-beta antibody
    see all

Images

  • ICC/IF image of ab155073 stained RAW 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab155073, 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-CEBP Delta antibody (ab155073) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Observed band size: 30 kDa (why is the actual band size different from the predicted?)


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab155073 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab155073 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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