Overview

  • Product nameAnti-CEBP Delta antibody
    See all CEBP Delta primary antibodies
  • Description
    Rabbit polyclonal to CEBP Delta
  • Specificityab65081 detects endogenous levels of total CEBP Delta protein.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CEBP Delta (C terminal).

  • Positive control
    • Extracts from LOVO cells and RAW264.7 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab65081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA 1/20000.

Peptide ELISA tested only.

ICC/IF Use at an assay dependent concentration.

Target

  • FunctionC/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses, may play an important role in the regulation of the several genes associated with activation and/or differentiation of macrophages.
  • Sequence similaritiesBelongs to the bZIP family. C/EBP subfamily.
    Contains 1 bZIP domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C/EBP delta antibody
    • C/EBP related protein 3 antibody
    • CCAAT/enhancer binding protein (C/EBP) delta antibody
    • CCAAT/enhancer binding protein delta antibody
    • CCAAT/enhancer-binding protein delta antibody
    • CEBP D antibody
    • CEBPD antibody
    • CEBPD_HUMAN antibody
    • CELF antibody
    • Crp 3 antibody
    • Crp3 antibody
    • NF IL6 beta antibody
    • NF-IL6-beta antibody
    • Nuclear factor NF IL6 beta antibody
    • Nuclear factor NF-IL6-beta antibody
    see all

Anti-CEBP Delta antibody images

  • Immunofluorescence analysis of murine spinal cord cells, staining CEBP Delta with ab65081 .

    Cells were fixed with acetone and blocked with 1% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/50 in 1% goat serum) for 16 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/250) ab150073) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-CEBP Delta antibody (ab65081) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells
    Lane 2 : Extracts from RAW264.7 cells
    Lane 3 : Extracts from RAW264.7 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 29 kDa

References for Anti-CEBP Delta antibody (ab65081)

This product has been referenced in:
  • Tong Y  et al. CEBPD Suppresses Prolactin Expression and Prolactinoma Cell Proliferation. Mol Endocrinol 25:1880-91 (2011). Read more (PubMed: 21980073) »
  • Younce CW  et al. MCP-1 (monocyte chemotactic protein-1)-induced protein, a recently identified zinc finger protein, induces adipogenesis in 3T3-L1 pre-adipocytes without peroxisome proliferator-activated receptor gamma. J Biol Chem 284:27620-8 (2009). WB ; Mouse . Read more (PubMed: 19666473) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Spinal cord)
Specification Spinal cord
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 21 2012

DISCOUNT CODE: XXXXXXXXXXX
Expiration date: 12-01-2013

I am very pleased to hear you would like to accept our offer and test ab65081 in IHC-P / IHC-Fr. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT1080)
Loading amount 100000 cells
Specification HT1080
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted May 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hepatocellular carcinoma)
Loading amount 40 µg
Specification Hepatocellular carcinoma
Treatment 5 mM/L of acetaldehyde for 24 Hours
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ratnakar Singh

Verified customer

Submitted Apr 02 2012

Thank you for contacting us. As you say, the Anti-CEBP Delta antibody (ab65081) has not as yet been assessed in IHC. However, unfortunately, due to the size of our catalogue we are unable to provide free samples to researchers due to the logistics invo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"