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Synthetic peptide corresponding to Human CEBP Delta (C terminal).
Our Abpromise guarantee covers the use of ab65081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
Peptide ELISA tested only.
|ICC/IF||Use at an assay dependent concentration.|
Immunofluorescence analysis of murine spinal cord cells, staining CEBP Delta with ab65081 .
Cells were fixed with acetone and blocked with 1% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/50 in 1% goat serum) for 16 hours at 4°C. An AlexaFluor®488-conjugated donkey anti-rabbit polyclonal IgG (1/250) ab150073) was used as the secondary antibody.